SH3 DOMAIN COMPLEXED TO NON NATURAL LIGAND

SH3结构域是在Signal中发现的小的模块化结构域 转导分子，并被认为参与了 蛋白质-蛋白质复合体。我们设计了基于多肽的， 从Sem5到SH3结构域的非天然配体。我们有 两种不同配体-SH3络合物的结构解析为2.2E。 然而，由于配体含有非天然氨基酸，具有不寻常的 侧链，我们希望获得更好的分辨率，以便 验证侧链几何图形和相互作用。

SH3 domains are small, modular domains found in signal transduction molecules and are believed to mediate the formation of protein-protein complexes. We have designed peptide-based, non-natural ligands to the SH3 domain from Sem5. We have the structures of two different ligand-SH3 complexes solved to 2.2 E. However, as the ligands contain unnatural amino acids with unusual sidechains, we would like to obtain better resolution in order to verify sidechain geometries and interactions.