STRUCTURE OF NUCLEAR PORE COMPLEX

(由霍华德·休斯医学院向G输入Blbel) HeLa细胞核孔复合体的胞质纤维为 贴着金子的标签。这些粒子出现在无数的短文中 难以确定其数量和轨迹的链条 二维视图。电子断层扫描被用来获得 这些信息。一个样本图像和一个10度倾斜系列是 从亚辛博士的实验室送来的。倾斜系列无法对齐 使用金色标签，因为倾斜间隔太大而无法跟随 单独的标记。根据《倾斜》系列制作了一部轮换电影 并送到亚辛博士那里。单倾斜系列是从一个 0.15(m节由亚辛博士发送。标本上待处理的区域 亚辛博士使用IVEM远程显微镜选择了IMAGE 网站。重建工作已经完成，可视化已经开始。

(Supported by Howard Hughes Medical Institute to G
nter Blbel) The cytoplasmic fibrils of the nuclear pore complex of HeLa cells are labelled with gold particles. The particles appear in numerous short chains whose number and trajectories are difficult to determine from two-dimensional views. Electron tomography is being used to obtain this information. A sample image and a 10-degree tilt series were sent from Dr. Yaseen's lab. The tilt series could not be aligned using the gold label because the tilt interval was too great to follow individual markers. A rotation movie was made from the tilt series and sent to Dr Yaseen. A single-tilt series was recorded from a 0.15(m section sent by Dr. Yaseen. The area on the specimen to be imaged was selected by Dr. Yaseen using the IVEM telemicroscopy website. The reconstruction was made and visualization has started.