SINGLE PHOTON COUNTING FLUORESCENCE & IMAGING APPARATUS

时间相关单光子计数(TCSPC)荧光 该仪器是一种最先进的光谱仪器。在……里面 符合在现有基础上开发新技术的目标 我们开发了一种时间分辨共聚焦光学仪器 给分子动力学和分子动力学研究带来革命性变化的光谱仪 结合空间耦合的光子过程 使用最先进的超快光谱方法进行分辨率测试。我们的 仪器旨在获取单一位置或扫描的图像 使用三种对比机制(力、强度和激发)的样品 状态生存期)来快速转换配置。在过去 去年，我们已将共焦显微镜更新为双通道操作 它同时允许检测两个独立的 光谱区域。这使我们能够监控 供体/受体对之间的共振荧光能量转移 许多不同的系统；最近的研究被标记为GCN-4 体内的多肽和胞内蛋白。其他发展 包括将传统的TCSPC系统与 共焦光学光谱仪。这种检测和检测的分离 电子学，这将有助于最大限度地使用这两种仪器，是 差不多完成了。仅更新和编程一个单独的 计算机数据采集中心(具有更快的处理器) TCSPC仍有待完成。

The time-correlated single photon counting (TCSPC) fluorescence apparatus performs as a state-of-the art spectroscopic instrument. In accord with the goal of developing new technologies out of existing instrumentation we have developed a time resolved confocal optical spectrometer which revolutionizes the study of molecular dynamic and photonic processes by incorporating the coupling the spatial resolution with state-of-the-art ultrafast spectroscopic methods. Our instrument is designed to acquire single location or scanned images of samples using three contrast mechanisms (force, intensity and excited state lifetime) in quickly converting configurations. Within the past year we have updated our confocal microscope to two channel operation which simultaneously allows detection of photons in two separate spectral regions. This allows us the capability of monitoring resonance fluorescence energy transfer between donor/acceptor pairs in many different systems; among those recently studies are tagged GCN-4 peptides and intracellular proteins in vivo. Other developments include the separation of the conventional TCSPC system from the confocal optical spectrometer. This separation in detection and electronics, which will facilitate maximum use of both instruments, is nearly complete. Only the updating and programming of a separate computer data acquisition center (with a faster processor) for the TCSPC remains to be completed.