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DOES MYOSIN HAVE CO EXISTING CONFORMATIONS?

DOES MYOSIN HAVE CO EXISTING CONFORMATIONS?
肌球蛋白是否具有共存的构象?
批准号:
6120273
负责人:
MANUEL F MORALES
金额:
$0.11万
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-03-01 至 2000-02-29

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中文摘要
翻译
N[4-(三氟甲基)苯基碘乙酰胺,“TPI”,已被 合成,并试图知道它的元素组成。这 组成,连同它的1-H和19-F光谱将用于 确定TPI的结构。可信的TPI将与 肌球蛋白的CyS-707,从而赋予肌球蛋白一个核磁共振(19-F)“报告” 一群人。希望这个小组将报告构象转变 被认为在Oo-100℃的温度范围内存在于肌球蛋白中。更多 准确的定位(更好的专用性)将通过 相应的(14-C)碘乙酰胺合成。
英文摘要
N[4-(Trifluoromethyl)phenylliodacetamide, "TPI", has been synthesized, and it is sought to know its elemental composition. This composition, together with its 1-H and 19-F spectra, will be used to confirm the structure of TPI. Authentic TPI will be reacted with Cys-707 of myosin, thus endowing myosin with an NMR (19-F) "reporter" group. Hopefully this group will report conformational transitions thought to occur in myosin in the Oo-10oC range of temperature. More accurate positioning (better specificity) will be acheived by a corresponding (14-C) iodoacetamide synthesis.
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