CALMODULIN TARGET PROTEINS; STRUCTURE FUNCTION RELATIONSHIPS

这项研究计划涉及检查不同的家庭， 钙调素依赖性酶，以确定：（1）结构或 （2）假定的功能基序的亲水性/疏水性指数 钙调素结合区;（3）磷酸化位点的位置 或假底物结构域或相对于该结构域的同源区， 钙调素结合域;（4）搜索数据库中的序列 与钙调素结合相关的区域相似;（5）次级 钙调蛋白（CAM）结合附近的结构预测 位点;（6）通过点图的内部重复序列;（6）检查 局部多重比对的动态规划程序。 酶 待研究的序列将包括CAM的整个谱 靶蛋白。 谱包括：1类靶蛋白， 与CAM结合而无相关的Ca 2+，对 Ca 2 +-CAM;在缺乏Ca 2 +-CAM的情况下强烈结合CAM的2类蛋白质 Ca 2+和被Ca 2+正激活的3类蛋白质， 对CAM-Ca 2+具有高亲和力，对类Ca 2+具有弱亲和力或没有亲和力。 2是巨噬细胞NO还原酶，肺环3 '，5'-核苷酸 磷酸二酯酶，百日咳博德特氏菌腺苷酸环化酶，和 糖原磷酸化酶B激酶;以及含有剩余的 酶和蛋白质如钙调神经磷酸酶，肌球蛋白轻链激酶， 钙调蛋白，腺苷酸环化酶，胞衬蛋白，IP 3激酶，marcks蛋白， 内收蛋白、蛋白激酶、Ca 2 + ATP泵和相关蛋白。

The research plan involves examining the different families of calmodulin-dependent enzymes to determine: (1) structural or functional motifs (2) hydrophilicity/hydrophobicity index of putative calmodulin-binding regions; (3) the position of phosphorylation sites or pseudosubstrate domains or the homologous regions relative to the calmodulin-binding domain; (4) searching database for sequences similar to regions associated with calmodulin binding; (5) secondary structure prediction in the vicinity of the calmodulin (CAM) binding site; (6) internal repeated sequences via dot plot; (6) examination of local multiple alignments by dynamic programming procedures. Enzyme sequences to be investigated will include the entire spectrum of CAM target proteins. The spectrum includes: Class 1 target proteins that bind to CAM without associated Ca2+ and have little affinity for Ca2+-CAM; Class 2 proteins that bind CAM strongly in the absence of Ca2+ and are positively activated by Ca2+ ; and Class 3 proteins that have high affinity for the CAM-Ca2+ and weak or no affinity for Class 2 are macrophage NO synthases, lung cyclic 3', 5'-nucleotide phosphodiesterase, bordetella pertussis adenylate cyclase, and glycogen phosphorylase b kinase; and class 3 containing the remaining enzymes and proteins such as calcineurin, myosin light chain kinase, caldesmon, adenylate cyclases, fodrin, IP3 kinase, marcks protein, adducins, protein kinases, Ca2+ ATPase pumps and related proteins.