课题基金 / 基金详情

MODULATION BASE SENSING

MODULATION BASE SENSING
调制基础传感
批准号:
6122899
负责人:
IGNACY GRYCZYNSKI
金额:
$3.4万
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-04-01 至 2000-03-31

项目摘要

项目成果

IGNACY GRYCZYNSKI的其他基金

相关文献

中文摘要
翻译
绿色荧光蛋白已被广泛用于基因研究 在细胞中表达,甚至在整个哺乳动物生物体中表达。 绿色荧光蛋白(GFP)的双光子诱导荧光 对突变体进行了观察和鉴定。没想到我们找到了 这种绿色荧光蛋白突变体的双光子截面非常高, 比野生型GFP高出数倍。他的一生和 用单光子和双光子激发测量了各向异性衰变 在全球范围内分析。双光子激发的零时间各向异性 比单光子激发大10/7倍。
英文摘要
Green fluorescent proteins has become widely used to study gene expression, in cells and even in whole mammalian organisms. Two-Photon induced fluorescence of Green Fluorescence Protein (GFP) mutant was observed and characterized. Unexpectedly we have found that two-photon cross-section of this GFP mutant is enormously high, several time higher than for wild type GFP. The lifetime and anisotropy decays were measured with one and two photon excitation and analyzed globally. The zero time anisotropy for two photon excitation is large than for one photon excitation by the factor 10/7.
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Ratiometric SPCE Detection of miRNA
  • 批准号:
    7671911
  • 项目类别:
  • 资助金额:
    $16.72万
  • 财政年份:
    2009
  • 负责人:
    IGNACY GRYCZYNSKI
  • 依托单位:
SPCE Imaging Microscopy
SPCE Imaging Microscopy
CONTROL OF FLUORESCENCE ANISOTROPY BY LIGHT QUENCHING FLUORESCENCE
  • 批准号:
    7181967
  • 项目类别:
  • 资助金额:
    $1.03万
  • 财政年份:
    2005
  • 负责人:
    IGNACY GRYCZYNSKI
  • 依托单位: