CORE--MOUSE PRODUCTION FACILITY

这一核心将为培育和跟踪所有 需要进行特殊突变(基因敲除)或转基因小鼠的 项目1-3中提出的实验。我们的前提是， 确保在3个项目中最有效地使用MICE(并确保 相互作用的项目使用的老鼠具有相同的 饲养和饲养的历史)，老鼠的饲养和饲养是最好的 由一个单独的设施执行，该设施将维护每个 努力协调它们的使用。通过从核心中提供小鼠 在哮喘中心内，每个科学项目都需要 老鼠可以专注于实现它们的基本科学目标。这个 核心将设在西蒙·C·消防员动物研究中心 设施。这个设施位于楼下一层 是AAAAAC和美国农业部完全认可的 并配备有合格的动物护理人员和有执照的 兽医。除了指导每个群体的繁殖 并在适当的情况下跟踪老鼠的基因类型，核心人员将 负责开展一定的实验前处理工作 协议。一旦养殖全面展开，核心人员 还将负责协调鼠标的使用和 将适当年龄和基因的小鼠分配给不同的 参与调查的人员。此外，这些人员将被 负责保存有关繁殖、健康、 和所有小鼠的基因类型，并确保这些记录是 对所有调查人员开放。通过这种方式，老鼠的适当的 将提供基因和年龄，以及统一高质量的产品 致所有调查人员。

This core will provide support for the breeding and tracking of all of the special mutant(knockout) or transgenic mice needed to conduct the experiments proposed in projects 1-3. Our premise is that, to ensure most efficient use of mice in the 3 projects (and to ensure that the interacting projects use mice which have an identical history of breeding and care), mouse care and breeding is best performed by a single facility, which will maintain colonies of each strain and coordinate their use. By providing the mice from a core within the Asthma center, each of the scientific projects requiring mice can concentrate on achieving their basic scientific goals. The Core will be located in The Simon C. Fireman Research Center Animal Facility. This facility, which is located on the floor below that of Drs. Kinet and GalIi, is fully accredited by AAAAAC and the USDA and is staffed by qualified animal care personnel and a licensed veterinarian. In addition to directing the breeding of each colony and tracking mouse genotypes where appropriate, Core personnel will be responsible for carrying out certain pre-experimental treatment protocols. Once breeding is underway at full scale, core personnel will also be responsible for coordinating the use of mice and distributing mice of appropriate age and genotype to the various participating investigators. Furthermore, the personnel will be responsible for keeping appropriate records on the breeding, health, and genotypes of all mice and making sure that these records are available to all investigators. In this way, mice of the appropriate genotype and age, and of uniformly high quality, will be available to all investigators.