ELECTROPHYSIOLOGY OF CANNABINOID ACTION ON RAT BEHAVIORS

这项研究计划旨在阐明基础神经元。 大麻滥用的中枢效应的潜在机制(S) 特别强调与计时有关的神经元过程， 感觉-运动整合，以及学习和记忆过程。一个 大量证据深刻地表明，大麻 干扰运动和计时反应，以及学习和记忆 人类的能力。使用一种新开发的技术，20-47单 现在可以在不同的位置同时记录神经元棘波序列 大鼠在各种行为任务中的大脑区域。《医学》 前额叶皮质、纹状体和黑质网状部 (NTR)是根据神经解剖学和 行为证据表明，这三个区域包含高密度的 大麻素受体和大麻素受体在运动和 所研究任务所需的认知功能。老鼠将会是 经训练以执行延迟匹配/不匹配样本(DMTS/DNMT)， 时间反应差异(TRD)、反应时(RT)和 听觉提示跑步机运动(TM)任务。DMTS是一项任务 旨在估计短期内涉及的认知过程 记忆和空间辨别功能。铁路发展局将研究 动物对主观时间流逝的估计能力 做出相应的运动反应。RT时间表测试了这种能力 对触发音做出反应，迅速从鼻塞上退出。TM 这项任务是将听觉音调和跑步机相结合来测试联系 在感觉提示(音调)和开始的运动功能之间。此外, 不同剂量大麻素对小鼠自发活动的影响 活动(从过度活动到惊厥对不同剂量的反应 )将被调查。剂量越来越大的 三角洲-9-四氢大麻酚(Delta-9-THC)，其活性成分 将对大麻进行测试(0.5毫克/公斤至10毫克/公斤)。这项调查 将决定神经元活动和行为表现的变化 在Delta9-THC中毒下。数据分析包括计算 行为时间事件周围的栅格和近震历史图。 脑内神经元之间的相关和同步活动 同一地区和跨不同地区。多人合奏活动 与行为事件有关的神经元将使用 判别分析。这项研究的目标是测试 大麻类物质选择性干扰成分的假说 皮质-纹状体系统内的信号。

This research proposal is aimed at elucidating the fundamental neuronal mechanism(s) underlying the central effects of marijuana abuse with special emphasis on the neuronal processes involved in timing, sensory-motor integration, and learning and memory process. A substantial amount of evidence has indicated marijuana profoundly disrupts locomotor and timing response, as well as learning and memory ability in human. Using a newly developed technique, 20-47 single neuronal spike trains can now be recorded concurrently in different brain areas of rats during a variety of behavior tasks. The medial prefrontal cortex (MPF), striatum and substantia nigra pars reticulate (NTR) are selected for investigation based on the neuroanatomical and behavioral evidence that these three areas contain a high density of cannabinoid receptor and are critically involved in locomotor and cognitive functions required for the tasks studied. Rats will be trained to perform delayed match/non-match to sample (DMTS/DNMTS), temporal response differentiation (TRD), reaction time (RT), and auditory-cued treadmill locomotion (TM) tasks. DMTS is the task designed to estimate the cognitive procedures involved in short term memory and spatial discrimination functions. TRD will examine the capability of the animal to estimate the passage of subjective time and to make a motor response accordingly. The RT schedule tests the ability of promptly withdrawing from nosepiece in response to trigger tone. TM is the task combining auditory tone and treadmill to test the linkage between a sensory cue (tone) and onset locomotor function. In addition, the effect of different doses of cannabinoid on spontaneous locomotor activity (from hyper-activity to catalepsy in response to different does of cannabinoid) will be investigated. An increasing dose of delta-9-tetrahydrocannabinol (delta-9-THC), the active component of marijuana,will be tested (0.5 mg to 10 mg/kg i.p.). The investigation will determine changes of neuronal activity and behavioral performance under delta-9-THC intoxication. Data analyses include computation of raster and perievent histrograms around behavioral time events. Correlationed and synchronized activities between neurons within the same region and across different regions. Ensemble activity of many neurons in relation to behavioral events will be examined using discriminant analysis. The goal of this research is to test the hypothesis that cannabinoids act to selectively disrupt component signals within the cortico-striatal system.