NERVE-MUSCLE INTERACTIONS DURNIG JAW MUSCLE DEVELOPMENT

描述(改编自研究人员摘要)：神经的作用 哺乳动物发育和分化过程中的肌肉相互作用 咀嚼肌肉和神经元组织的模式 几乎没有受到关注。然而，许多研究都集中在 神经-肌肉相互作用在肢体肌肉发育中的作用。上一首 对肢体的研究已经确定运动神经元和它们的肌肉 神经的正常发育是相互依赖的，他们的 维护和它们的生理特性。尽管有价值的线索 神经-肌肉相互作用在发育和维持中的作用 可以从对肢体肌肉的研究中收集到咀嚼肌肉的 肢体研究的结果可能并不完全适用。中国人的肌肉 咀嚼与四肢肌肉的胚胎起源不同， 支配它们的感觉神经元和运动神经元以及 成体表达的纤维类型。此外，对 需要参与口面部形态发生的发育过程 对成人的形态和功能的了解，并可能提供有价值的 洞察在修改成人时可能遇到的限制 形式。因此，这项建议的重点是调查 咀嚼肌和三叉神经的时空发育 复杂性，以加强我们对神经-肌肉相互作用的理解 发展。在这个项目中，提出了两个要检验的假设。 并专注于三叉神经中枢神经系统组织，咀嚼 肌肉发育和神经-肌肉相互作用。第一个假设是 三叉神经系统的中枢成分参与神经支配 包括MotV和MesV在内的咀嚼肌肉的发育是不同的 时间空间序列，并响应来自其目标的线索。这个 第二个假设是，成人咀嚼肌纤维的表型 每块颌肌内的肌纤维分布取决于 三叉神经-肌肉相互作用。这两个假设将得到检验。 包括体内和体外研究在内的三个具体目标。

DESCRIPTION (Adapted from investigator's Abstract): The role of nerve muscle interactions in the development and differentiation of mammalian muscles of mastication and the patterning of neuronal organization has received little attention. However, much research has concentrated on the role of nerve-muscle interactions in limb muscle development. Previous studies in the limb have determined that motoneurons and the muscles they innervate are dependent on one another for their normal development, their maintenance and their physiological properties. Although valuable clues as to the role of nerve-muscle interactions in the development and maintenance of masticatory muscles can be gleaned from studies of limb muscle, the result of limb studies may not be totally applicable. The muscles of mastication differ from limb muscle in their embryonic origin, the origin of the sensory and motor neurons innervating them and in the uniqueness of the fiber types expressed in the adult. Moreover, an understanding of developmental processes involved in orofacial morphogenesis is required for an understanding of the adult form and function, and may provide valuable insight into what limitations might be encountered in modifying the adult form. Therefore, the focus of this proposal is to investigate the temporospatial development of the masticatory muscles and the trigeminal complex to enhance our understanding of nerve-muscle interactions during development. Two hypotheses are proposed to be tested during this project and focus on trigeminal central nervous system organization, masticatory muscle development and nerve-muscle interactions. The first hypothesis is that central components of the trigeminal system involved in the innervation of the muscles of mastication including motV and mesV, develop in distinct temporospatial sequences and in response to cues from their targets. The second hypothesis is that the adult phenotype of masticatory muscle fibers and the muscle fiber distribution within each jaw muscle are dependent on trigeminal nerve-muscle interactions. These two hypotheses will be tested in three specific aims that include in vivo and in vitro investigations.