CORE--VIROLOGY FACILITY

病毒学核心病毒学核心的目的是为小鼠病毒感染的进行提供设施和技术援助。生物安全三级（BL 3）生物防护套间内的一个房间将被分配用于本项目的工作。研究技术人员将负责对小鼠进行病毒接种、后续监测和监控，以及小鼠放血和组织收获，然后将其分发给其他项目实验室或形态学核心实验室。该病毒学核心还将负责病毒的生长、制备、纯化和测定，用于产生抗病毒抗体，以及用于维持LCMV特异性T细胞转基因小鼠。它将制备负载肽的IgG-MHC二聚体，以定量病毒特异性T细胞，并将进行一些抗病毒CTL测定，T细胞增殖测定，产嘌呤细胞测定和有限稀释测定，以监测T细胞频率，特异性和功能。

VIROLOGY CORE The purpose of the Virology Core is to provide facilities and technical assistance for the conduction of virus infections in mice. A room within a biosafety level three (BL3) Biocontainment Suite will be assigned for work on this project. A research technician will be responsible for the inoculation of mice with viruses, for their subsequent surveillance and monitoring, and for the bleeding of mice and harvesting of tissues, which may then be distributed to the other Project Laboratories or to the Morphology Core Lab. This Virology Core will also be responsible for the growth, preparation, purification, and assay of viruses, for the generation of antiviral antibodies, and for the maintenance of LCMV- specific T cell transgenic mice. It will prepare peptide-loaded IgG-MHC dimers to quantify virus-specific T cells, and it will perform some of the antiviral CTL assays, T cell proliferation assays, cytokine-producing cell assays, and limiting dilution assays to monitor T cell frequency, specificity, and function.