CORE--SIMIAN

猿核将负责所有的猴子操作和 席夫病毒学。因此，它提供了必要的试剂， 完成本建议书中所有项目的目标。这个核心 将获得猴子，用滴定的HIV/DeltaB670感染它们， 使用PMPA进行治疗，并使用提供的疫苗进行免疫 按照拟议的时间表按项目1和项目2分列。猴子将会是 经临床检查有无免疫缺陷信号， T细胞亚群的计数和血浆病毒载量的定量。这个 这些化验的结果将被组织到计算机数据库中，使用 铁匠软件和一个与该项目联网的太阳火花站 实验室，便于随时取用。这个核心将为临床提供 在这个项目上照顾猴子，并将负责收集 并将血液、淋巴和骨髓样本分配给 其他需要的项目。处于终末期疾病的猴子或处于 实验的终止将被人道地牺牲和组织 收集并分发到项目实验室进行分析。

The simian core will be responsible for all monkey manipulations and the SIV virology. As such, it provides the necessary reagents required for the accomplishment of the aims of all the projects in this proposal. This core will obtain monkeys, infect them with a titered stock of HIV/DeltaB670, administered treatment with PMPA, and immunize with the vaccines provided by projects 1 and 2 according to the proposed schedule. Monkeys will be followed for signals of immunodeficiency by clinical examination, enumeration of T cell subsets, and quantitation of plasma virus loads. The results of these assays will be organized in a computer database using Blacksmith software and a Sun sparkstation networked to the project laboratories for ready access. This core will provide for the clinical care of monkeys on this project and will be responsible for the collection and distribution of blood, lymph node, and bone marrow samples to the other projects as needed. Monkeys in end-stage disease or at the termination of the experiment will be humanely sacrificed and tissues collected and distributed to project laboratories for analysis.