CORE--MOLECULAR BIOLOGY

Tssue文化核心设施对此至关重要 计划项目，因为他们将继续提供来自 受EB影响的个人，来自其未受影响的家庭成员，以及 在所有项目中从无关的健康对照中进行研究。在.期间 在前几年的支持下，组织培养设施已经处理了 从EB和对照受试者中获得大量人类细胞。它还 维持了大量自发或通过病毒变形的 对阐明基因有极大帮助的人类细胞系 BMZ大分子的表达，包括III型胶原。这些 已建立的细胞系包括人羊膜内皮细胞系 (Wish)、人表皮样口腔癌细胞系(KB)和人 纤维肉瘤细胞系(HT-1080) (ATCC)。

The Tssue Culture Core facilities are of central importance for this Program Project, as they will continue to provide cultured cells from individuals affected with EB, from their unaffected family members, and from unrelated healthy controls for study in all Projects. During the previous years of support, the tissue culture facility has handled a large number of human cells from EB and control subjects. It has also maintained a large number of either spontaneously or virally transormed human cell lines that have been extremely helpful in elucidating gene expression of BMZ macromolecules, including type VII collagen. These established cell lines include a human amniotic endothelial cell line (WISH), a human epidermoid oral carcinoma cell line (KB), and a human fibrosarcoma cell line (HT-1080), which are commercially available (ATCC).