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POLYMORPHIC UPSTREAM STRUCTURE & PFG 27/25 GENE CONTROL

POLYMORPHIC UPSTREAM STRUCTURE & PFG 27/25 GENE CONTROL
多态上游结构
批准号：
6170096
负责人：
Ute Frevert
金额：
$33.65万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
项目状态：
已结题
起止时间：
1998-05-01 至 2002-04-30
来源：
https://reporter.nih.gov/project-details/6170096
关键词：
Plasmodium falciparum developmental genetics gene expression genetic mapping genetic polymorphism genetic transcription microorganism culture microorganism genetics nucleic acid hybridization nucleic acid probes nucleic acid sequence polymerase chain reaction protein structure pulsed field gel electrophoresis regulatory gene sex differentiation southern blotting transfection

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中文摘要
翻译
描述(改编自申请者摘要)：疟原虫 恶性疟原虫极早配子体阶段基因Pfg27/25具有多态 被保守包围的编码序列的基因座上移 序列。多态区域的结构指定精确或 改变了该基因的血液阶段特异性转录。多态的 结构产生于一种尚不具有特征的DNA重排。这个 这项提案的总体目标是确定详细的结构 在显示不同血相的实验室分离株中该基因座的 Pfg17/25基因的特定转录模式，以及 这些多态在自然界中都会发生。Pfg27/25的5‘末端 还将确定mRNAs以查看转录起始位置 相对于多形域的边界发生。
英文摘要
DESCRIPTION (Adapted from the Applicant's Abstract): The Plasmodium falciparum very early gametocyte stage gene, Pfg27/25, has a polymorphic locus upstreasm of the coding sequences which is surrounded by conserved sequences. The structure of the polymorphic region specifies accurate or altered blood stage specific transcription of the gene. The polymorphic structures arise by an as yet uncharacterizes DNA rearrangement. The overall objectives of this proposal are to determine the detailed structure of this locus in laboratory isolates that display different blood satge specific transcription patterns for the Pfg17/25 gene, and the extent to which these polymorphisms occur in nature. The 5' terminus of the Pfg27/25 mRNAs will also be determined to see where the transcription start sites occur with respect to the boundaries of the polymorphic domain.
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Development of a topical malaria vaccine.
The Lung and Malaria
The Lung and Malaria
Imaging preerythrocytic Plasmodium stages in naive and immune individuals
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