ROLE OF SRC 1 IN SEXUAL DIFFERENTIATION OF RAT BRAIN

描述(申请人摘要)：对细胞过程的理解 在围产期影响大脑的性别分化 很重要，因为它们会对正常行为产生持久的影响 动物的生理学。围产期性腺激素暴露 经期可以使大脑男性化，也可以女性化。因为性腺激素影响 脑发育与细胞内的类固醇受体结合 了解类固醇激素是如何与大脑相互作用的 类固醇受体影响基因组活性并最终影响大脑 发展。最近的研究报道，类固醇受体与 基因转录所必需的其他蛋白质。一种这样的蛋白质， 被称为类固醇受体辅活化子-1(SRC-1)，已知对 然而，类固醇在体外制剂中的作用尚不清楚SRC-1是否是 在大脑中进行类固醇活动所必需的。在目前的一系列 实验，我们将确定SRC-1的表达是否在 并确定SRC-1蛋白是否需要 类固醇在大脑中的作用。

DESCRIPTION (applicant's abstract): An understanding of the cellular processes that influence sexual differentiation of the brain during the perinatal period is important as they have enduring consequences on the normal behavior and physiology of an animal. Exposure to gonadal hormones during the perinatal period can either masculinize or feminize the brain. As gonadal hormones affect brain development by binding to intracellular steroid receptors located within the brain, it is important to understand how steroid hormones interact with steroid receptors to influence genomic activity and ultimately brain development. Recent studies have reported that steroid receptors interact with other proteins that are necessary for gene transcription. One such protein, called steroid receptor coactivator-1 (SRC-1), is known to be obligatory for steroid action in in vitro preparations however, it is not known if SRC-1 is required for steroid action within the brain. In the present series of experiments, we will determine if SRC-1 expression is sexually dimorphic within the brain during development and determine if SRC-1 protein is required for steroid action within the brain.