DOUBLE-STRANDED RNA CONDENSATION BY MULTIVALENT CATION

dsRNA是基因组包装中的生物学机制，因其在非病毒基因传递领域的重要性而受到了广泛的关注。然而，尽管在某些病毒中包装基因组dsRNA或将常见单链RNA中的dsRNA区域与蛋白质结合，但dsRNA缩合却完全缺乏关注。该电子显微镜的主要目的是跟踪缩合动力学和聚合形态。配体结构和迁移率对dsRNA缩聚的影响也将使用通常用于缩聚dsDNA的阳离子配体进行研究。电介质常数对dsRNA冷凝的影响将通过水-酒精混合物来确定螺旋电荷密度和刚度的作用。该项目的最终目标是确定dsDNA和等高dsRNA片段的比较。

DESCRIPTION The objective of this proposed project is the elucidation of the mechanism of double-stranded (ds) RNA condensation dsRNA, the biological mechanism in genome packaging, has received a significant amount of attention because of its importance in the field of non-viral gene delivery. Yet, dsRNA condensation has received a complete lack of attention has a complete lack of attention despite the packaging of genomic dsRNA in certain viruses or associations of dsRNA regions in commonly found single-stranded RNA with proteins. A primary goal of this electron microscopy will be used to follow the condensation kinetics and aggregate morphologies. The effects of ligand structure and mobility on dsRNA condensation will also be studied using cartionic ligands commonly used to condense dsDNA. The effect of dielectric constant on dsRNA condensation will be addressed using water-alcohol mixtures to determine the roles of helical charge density and stiffness. The final goal of this project is a definitive comparison of dsDNA and dsRNA fragment of equal contour.