CELLULAR-BEHAVIORAL ANALYSIS OF SHORT & LONG-TERM MEMORY

这是根据ADAMHA Research提出的五年支持请求 科学家发展奖计划。这样做的长期目标是 研究项目是分析AN的细胞和分子机制。 联想学习和记忆的例子。在这篇文章中提出的研究 Grant将提供对内存基础的一般原则的见解 一次试验后的联想体验，并做出贡献 为了阐明对记忆转换至关重要的事件 变成一种更持久的形式，长期记忆。为了实现这些目标， 联想学习和记忆的多学科研究 将在海洋软体动物Hermissanda中进行，这一准备工作 已被证明在学习的生物物理学和分子研究中很有用。这个 研究将结合使用细胞神经生理学、 生物物理、生化和行为技术，以研究短期和 完整的和孤立的神经系统的长期记忆。一个主要目标 这项研究的重点是研究蛋白质的贡献。 磷酸化到诱导背后的依赖时间的过程， 短期和长期记忆的维持和表达。最近的 用广谱激酶抑制蛋白激酶C的发现 抑制剂或通过下调来耗尽激酶水平，阻断 短期记忆的诱导，但不是长期记忆的诱导，提供了额外的 有证据表明这两个阶段可能存在不同的分子机制 对记忆的记忆。提出了进一步研究解离的建议 在短期和长期记忆和蛋白质的贡献之间 激酶和蛋白质的磷酸化可能对诱导起作用， 不同记忆阶段的维护和表达。第二个目标是 这个项目是为了分析特定的膜电导，这些电导 通过一次试验条件作用进行修改，并确定 增强的兴奋性和突触强度的改变 长期记忆的表现。第三个主要目标是确定 神经回路的潜在运动和发展半完整 运动神经元同步记录和测量的准备 对行为的条件性改变。这些研究应该有助于 阐明短期和长期记忆的基本机制以及这种记忆是如何 其机制可能与临床记忆障碍有关。

This is a request for five years of support under the ADAMHA Research Scientist Development Award Program. The long-term objective of this research project is to analyze the cellular and molecular mechanisms of an example of associative learning and memory. The studies proposed in this grant will provide insights into general principles underlying memory for an associative experience following one-trial conditioning, and contribute to the elucidation of the events critical for the transformation of memory into a more enduring form, long-term memory. Toward these goals, a multidisciplinary approach to the study of associative learning and memory will be carried out in the marine mollusc Hermissenda, a preparation that has proven useful in biophysical and molecular studies of learning. The research will use a combination of cellular neurophysiological, biophysical, biochemical, and behavioral techniques to study short and long-term memory in intact and isolated nervous systems. One primary goal of the research is an investigation of the contribution of protein phosphorylation to the time-dependent processes underlying the induction, maintenance and expression of short- and long-term memory. The recent discovery that inhibiting protein kinase C with broad spectrum kinase inhibitors or depleting kinase levels by down-regulation, blocks the induction of short-term, but not long-term memory provides additional evidence that different molecular mechanisms may underlie these two phases of memory. Studies are proposed to further investigate the dissociation between short and long-term memory and the contribution that protein kinases and protein phosphorylation may make to the induction, maintenance, and expression of different memory stages. A second goal of this project is to analyze the specific membrane conductances that are modified by one-trial conditioning, and identify the contribution of enhanced excitability and modifications in synaptic strength to the expression of long-term memory. The third major goal is to identify the neural circuitry underlying locomotion and develop a semi-intact preparation for simultaneously recording from motor neurons and measuring conditioned modification of behavior. These studies should help to elucidate basic mechanisms of short and long-term memory and how such mechanisms may be related to clinical disorders of memory.