DEVICES FOR HIGH THROUGHPUT CELLULAR PROTEIN ANALYSIS

该项目的目标是开发用于将细胞蛋白质综合到正常、癌前和癌组织中的微加工装置。 具体来说，我们建议开发芯片实验室技术，作为目前用于全面蛋白质分析的缓慢和劳动密集型二维（2D）凝胶方法的替代方案。 微芯片将集成在一个单一的结构元素，使多维分离的蛋白质混合物，无论是芯片上标记的荧光检测或电喷雾电离（ESI）的分析物直接，在线接口与质谱（MS）。 该项目的成功完成将导致高通量，自动化设备用于识别疾病标志物和阐明细胞转化的分子基础。 这些设备将有研究应用，了解细胞从正常到疾病状态的转变。 此外，这些研究工具也将在临床诊断和治疗开发中得到应用。

The objective of this project is to develop microfabricated devices for the comprehensive of cellular proteins to normal, precancerous and cancerous tissues. Specifically, we propose to develop lab-on-a-chip technologies as an alternative to the slow and labor-intensive two dimensional (2D) gel methods currently used for comprehensive protein analysis. The microchips will integrate on a single structure elements that enable multidimensional separations of protein mixtures, with either on-chip labeling for fluorescence detection or electrospray ionization (ESI) of the analytes for direct, on line interfacing with mass spectrometry (MS). Successful completion of the project will result in high throughput, automated devices for use in identifying disease markers and elucidating the molecular basis of cellular transformation. These devices will have research applications for understanding cellular transitions from normal to diseased states. In addition, these research tools will also find application in clinical diagnosis and therapy development.