RAT GENOME DATABASE

大鼠是生理学、药理学和毒理学研究中应用广泛、价值很高的模型系统。美国和国际大鼠基因组计划开发了新的基因组工具和资源。通过这些努力开发的信息和试剂以惊人的速度增长。超过8000个遗传标记覆盖了大鼠基因组，数百个已知基因被置于这个遗传框架内。多个大插入基因组文库、辐射杂交（RH）细胞系和图谱、标准化cDNA文库、48个自交系遗传标记的等位基因鉴定、细胞遗传学图谱、超过90,000个ESTs（其中2,000个已绘制）和比较图谱已经生成。然而，对数据库基础设施的支持是有限的。目前，数据分布在许多研究实验室的许多数据库中，许多重要的数据集在互联网上是不可用的。我们提出以下具体目标：解决社区范围内对目前可用的公开大鼠基因组数据库的直接需求（http://www.informatics.jax.org/rat），while)，我们将过渡到RGD。2. 在RGD中填充以下区域的数据：ESTs、基因、标记物、引物名称、遗传图谱和比较图谱以及支持的同源性信息（TJL）和UniGene簇。实施RGD管理系统和支持的数据结构，允许项目人员直接输入和编辑数据，并从基因组中心下载大量数据。4. 提供大鼠RH参考图谱，该图谱与EST和遗传标记的集合相结合。5. 提供使用大鼠基因组资源的在线工具。6. 通过提供用户培训和联机帮助、答复用户查询以及开展社区外展活动（如访问科学家培训方案），促进将RGD作为数据分析和探索的工具。

The rat is a widely-used and highly-valued model system for physiology, pharmacology, and toxicology. The US and International Rat Genome Projects have developed new genomic tools and resources. The information and reagents developed through these efforts has grown at an astounding pace. More than 8,000 genetic markers cover the rat genome, and hundreds of known genes are placed within this genetic framework. Multiple large- insert genomic libraries, radiation hybrid (RH) cell lines and map, normalized cDNA libraries, allele characterization for genetic markers in 48 inbred strains, a cytogenetic map, over 90,000 ESTs (2,000 of them mapped), and comparative maps have been generated. However, the support for the database infrastructure has been limited. Currently, data are distributed across many databases at scores of research labs, and many important data sets are not available on the internet. We propose the following specific aims: 1. Address the immediate community-wide need for a publicly available rat genomic database currently available (http://www.informatics.jax.org/rat),while we transition to RGD. 2. Populate the RGD with data in the following areas: ESTs, genes, markers, primer names, genetic maps and comparative maps and supporting homology information (TJL), and UniGene Clusters. 3. Implement the RGD curation system and supporting data structures to allow direct data entry and editing by project staff and downloads of bulk data from genome centers. 4. Provide a rat RH reference map that is integrated with collections of EST and genetic markers. 5. Provide on-line tools for using the resources of the rat genome. 6. Promote the use of RGD as a tool for data analysis and exploration by providing user training and on-line help, by responding to user queries, and by community outreach activities, such as a visiting scientists training program.