CORE--BIOCHEMISTRY AND MOLECULAR BIOLOGY FACILITY

为了给研究项目提供核心支持， 助学金计划有四个核心单位，即：管理核心（P.I.; D.N.格兰杰），细胞培养核心（P.I.; P.R Kvietys），生物化学核心 （P/I/; M. B. Grisham）和形态学核心（P.I.; R. Specian）。 这些 Cores将为研究人员提供共享设施和资源， 在加强各组织之间的互动方面发挥着重要作用 investigators. 行政核心的主要职能是： 协调项目和核心组件的活动，以管理 采购和预算事项，为秘书处提供秘书处支助， 各种项目和核心，并安排会议和研讨会， PPG。 细胞培养核心将提供培养的内皮细胞和 PPG中特定项目的上皮细胞。 生物化学核心 将进行各种分光光度和荧光分析，蛋白质 分离和纯化，和酶灭活程序，为几个 项目 形态学核心将提供设施和专业知识， 对粘膜、血液和血液的光学和电子显微镜研究 血管、白细胞和培养的内皮细胞。 四个核心单元 将提供一种有效的手段来产生所需的服务， 进行拟议的研究，并实现既定的目标， 程序.

In order to provide core support for the research projects, the Program Project Grant has four Core units, i.e., an Administrative Core (P.I.; D.N.Granger), a Cell Culture Core (P.I.; P.R Kvietys), a Biochemistry Core (P/I/; M.B. Grisham), and a Morphology Core (P.I.; R. Specian). These Cores will provide shared facilities and resources for the investigators in the PPG and play an important role in enhancing the interaction among investigators. The primary functions of the Administrative Core are to co- ordinate the activities of the component Projects and Cores, to manage purchasing and budgetary matters, to provide secretarial support for the various Projects and Cores, and to schedule meetings and seminars in the PPG. The cell Culture Core will provide cultured endothelial and epithelial cells for specific Projects in the PPG. The Biochemistry Core will perform various spectrophotometric and fluorometric assays, protein separation and purification, and enzyme inactivation procedures for several projects. The Morphology Core will provide facilities and expertise for light and electron microscopic studies of the mucosal membrane, blood vessels, leukocytes, and cultured endothelial cells. The four Core units will provide an efficient means for generating the services needed to conduct the proposed research and to attain the stated goals of the Program.