PATHOGENESIS AND PREVENTION OF AUTOIMMUNE DIABETES

本申请是为一项方案项目赠款提供新资金 自身免疫性糖尿病的发病机制及预防这个 这笔资金的目的是让四名工作过的调查人员 一起在过去继续做未来的事情。该计划 由三个核心支持的四个项目组成。这些项目包括 如下：项目1：C.A.Janeway，Pi.本项目重点关注CD8 T 可以将急性糖尿病转移给NOD小鼠的细胞。这些克隆的CD8 T细胞株在三个小时内进入胰岛并导致全部糖尿病 对老鼠进行了测试。我们将研究它们的归宿，构建TCR转基因 小鼠，并鉴定他们检测到的β细胞抗原。项目2：R.A. Flavell，Pi.此项目旨在分析模型中的公差 使用正常和NOD转基因小鼠，询问是否有 NOD小鼠的一般变位耐受性，并测定其 机制。项目3：A.C.Hayday，Pi.这个项目将检查 自身抗原谷氨酸脱羧酶(GAD)在血管紧张素转换酶中的作用 点头老鼠。我们观察到胰岛素炎的强烈促进和一些 这些小鼠患糖尿病的几率增加。该项目的目的是研究 这种加速，使用针对GAD的TCR转基因。项目4： 作者：R.Sherwin，Pi.这个项目主要集中在 胰岛素特异性保护性T细胞克隆的特性。他们的 作用机制及其发展将用 TCR转基因技术，有望研发出疫苗。四个项目 由三个核心设施支持：核心A、管理核心、 C.Janeway Pi；核心B，动物核心，F.王，圆周率；C芯，小岛 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容

The present application is for new funding of a program project grant on the Pathogenesis and Prevention of Autoimmune Diabetes. The purpose of this funding is to allow four investigators who have worked together in the past to continue to do in the future. The program consists of four projects supported by three cores. the projects are as follows: Project 1: C.A. Janeway, PI. This project focuses on CD8 T cells that can transfer acute diabetes to NOD mice. These CD8 cloned T cells lines enter the islets in three hours and cause diabetes in all mice tested. We will study their homing, construct a TCR transgenic mouse, and identify the beta cell antigen they detect. Project 2: R.A. Flavell, PI. This project is designed to analyze tolerance in models using normal and NOD transgenic mice, to ask if there is a generalized defecting tolerance in the NOD mice, and to determine its mechanism. Project 3: A.C. Hayday, PI. This project will examine the role of the autoantigen glutamic acid decarboxylase (GAD) in NOD mice. We have observed strong promotion of insultis and some increase in diabetes in these mice. The aim of the project is to study this acceleration, using TCR transgenes specific for GAD. Project 4: RS.Sherwin, PI. This project is mainly focused on the characterization of insulin-specific protective T cell clones. Their mechanism of action and their development will be characterized using TCR transgenics, hopefully leading to a vaccine. The four projects are supported by three core facilities: Core A, Administrative Core, C. Janeway PI; Core B, Animal core, Fs. Wong, PI; Core C, Islet Isolation and Imunohistopathology Core, M. Solimena, PI.