PROTEIN/PEPTIDE AMINO ACID SEQUENCER

这项申请是科罗拉多州立大学(CSU)从事蛋白质结构和功能研究的研究人员共同努力的结果，目的是为购买最先进的自动蛋白质测序仪(PE应用生物系统过程模型)获得资金。该仪器将由大分子资源设施(MRF)容纳和操作，该设施是CSU的一个共享仪器项目，其中包括一个蛋白质核心实验室。埃德曼测序技术已经成为MRF蛋白质结构分析的重要工具。它与质谱学方法协同使用，以完成涉及蛋白质鉴定、从头测序、蛋白质翻译后修饰的表征以及其他蛋白质/结构研究的项目。新的测序仪将取代旧的测序仪(PE-应用生物系统型号473A型)，该测序仪在多年的生产性使用后已过时，主要原因是制造商缺乏可用的更换部件。新仪器将提供更高的灵敏度、吞吐量和可靠性。主要用户群体将利用现有的基础设施，通过将仪器放入最大残留限量实验室，提供必要的专门知识和可访问性。将以先到先得的方式向主要用户群体提供最优先的服务，并收取仪器操作和维护费用。学术界内的其他潜在用户将能够以次要优先顺序使用该仪器。提交这项建议的研究人员的研究兴趣涉及广泛的基本生物学和健康相关问题，包括：传染病、疫苗和药物开发；转录调节和染色质结构；神经元细胞骨架动力学和信号转导；以及氨基酸代谢。这一用户群体和CSU扩展的研究社区的研究兴趣的多样性将确保这次购买的好处将在公共卫生领域产生深远影响。

This application is a joint effort of investigator sat Colorado State University (CSU) who engage in the study of protein structure and function, to obtain funding for the purchase of a state-of-the-art automated protein sequencer (PE Applied Biosystems Procise Model). The instrument will be housed and operated by Macromolecular Resource Facility (MRF) which is a shared instrumentation program at CSU that contains a protein core laboratory. Edman sequencing technology has been an essential tool for protein structure analysis at MRF. It is used synergistically with mass spectrometry methods to complete projects involving identification of proteins, de novo sequencing, characterization of post-translational modifications in proteins, and other protein/structure studies. The new sequenator will replace an old sequencer (PE-Applied Biosystems Model 473A) which after many years of productive use has become obsolete mainly due to the lack of available replace parts from the manufacturer. The new instrument will provide higher sensitivity, throughput and reliability. The major user group will take advantage of an established infrastructure that provides the necessary expertise and accessibility by placing the instrument in the MRF laboratory. Service will be offered with top priority, on a first come first served basis, to the major user group with a charge to cover the cost of instrument operation and maintenance. Other potential users within the academic community will be able to access the instrument on a secondary priority. The research interests of the investigators submitting this proposal cover a wide range of basic biological and health related problems including: infectious diseases, vaccine and drug development; transcriptional regulation and chromatin structure; neuronal cytoskeleton dynamics and signal transduction; and amino acid metabolism. The diversity of research interests of this user group and the extended research community at CSU will ensure that the benefits of this purchase will be far reaching in the areas of public health.