ELECTRON PARAMAGNETIC RESONANCE SPECTROMETER

该提案为多功能x波段/ q波段电子顺磁共振（EPR）光谱仪寻求资金。主要用户包括Judith Klinman教授、James McCusker教授、Kenneth Raymond教授、Kenneth Sauer教授、David Wemmer教授和dr。劳伦斯伯克利国家实验室物理生物科学部的Melvin Klein和Vittal Yachandra。这组科学家的研究兴趣跨越金属酶化学，生物无机化学，电子转移，神经生物化学和光合作用。本提案描述了在这些领域中，EPR光谱如何在化学和生化系统的识别和表征中发挥重要作用，以及帮助阐明光诱导电子转移过程中蛋白质功能和电子转移途径的机制。例如，Klinman教授的研究涉及广泛的含铜和含铁酶的活性机制。EPR光谱既可以用来表征酶的金属基活性位点，也可以用来识别所研究的整个生化过程中的中间体。绍尔教授和博士。Klein和Yachandra研究光系统II的氧气进化复合体，广泛使用EPR光谱来帮助表征参与将h2o转化为O2的物种。McCusker教授的研究涉及电子转移蛋白等多核团簇中电子交换和电子转移之间的关系。EPR可用于识别电子转移光产物以及帮助表征团簇本身的电子结构。Raymond教授的研究领域还包括生物无机化学，EPR光谱是表征铁载体以及超分子组装中原子间相互作用的重要技术。最后，Wemmer教授对朊病毒蛋白结构动力学的研究可以利用EPR光谱来确定蛋白质的结构，利用未配对自旋可以相互作用的长距离优势，补充他的核磁共振技术提供的局部和短程结构信息。所有这些小组显然都需要常规使用EPR光谱仪，但加州大学伯克利分校却没有这样的仪器。因此，本提案寻求为购置最先进的变温光谱仪（1.8 - 400 K）以及x波段（约9千兆赫）和q波段（35千兆赫）能力提供资金。我们相信，该研究所的这项投资不仅将大大提高主要用户群体的研究，而且将大大提高加州大学伯克利分校化学系的整体研究水平。

This proposal seeks funding for a versatile X-band/Q-band electron paramagnetic resonance (EPR) spectrometer. The group of major users consists of Professors Judith Klinman, James McCusker, Kenneth Raymond, Kenneth Sauer, David Wemmer, and Drs. Melvin Klein and Vittal Yachandra of the Physical Biosciences Division of the Lawrence Berkeley National Laboratory. The research interests of this group of scientists span metalloenzyme chemistry, bioinorganic chemistry, electron transfer, neurobiochemistry, and photosynthesis. This proposal describes how, in each of these areas, EPR spectroscopy can play a vital role in the identification and characterization of chemical and biochemical systems, as well as helping to elucidate mechanisms of protein function and electron transfer pathways in photo-induced electron transfer processes. For example, Professor Klinman's research is concerned with the mechanism of activity in a wide range of Cu- and Fe-containing enzymes. EPR spectroscopy can be utilized both to characterize the metal-based active site(s) of the enzyme as well as identify intermediates in the overall biochemical process being studied. Professor Sauer and Drs. Klein and Yachandra work on the oxygen-evolving complex of photosystem II, using EPR spectroscopy extensively to help characterize species involved in the conversion of H20 to O2. Professor McCusker's research concerns the correlation between electron exchange and electron transfer in polynuclear clusters such as those found in electron transfer proteins. EPR can be used to identify electron transfer photoproducts as well as help to characterize the electronic structures of the clusters themselves. Professor Raymond's research is also in the area of bioinorganic chemistry, where EPR spectroscopy is an important technique for the characterization of Fe siderophores, as well as interatomic interactions in supramolecular assemblies. Finally, Professor Wemmer's research on the structural dynamics of the prion protein can utilize EPR spectroscopy to determine the structure of the protein by taking advantage of the long distance over which unpaired spins can interact, complimenting the local and short-range structural information afforded by his NMR techniques. All of these groups have a clear need for routine access to an EPR spectrometer, yet the University of California at Berkeley does not have such an instrument. This proposal therefore seeks funding for the acquisition of a state-of-the-art spectrometer with variable-temperature (1.8 - 400 K) as well as both X-band (ca. 9 GHz) and Q-band (35 GHz) capabilities. We believe that this investment on the part of the Institute will greatly enhance not only the research of the major user group, but of the Department of Chemistry at UC Berkeley as a whole.