BIACORE 2000 PROCESSING UNIT

S提出利用表面等离子体共振对多种生物系统中特定的蛋白质-蛋白质和蛋白质-核酸相互作用进行动力学和热力学分析。RAJBANDARY实验室将在真细菌和真核生物中进行蛋白质合成启动的研究。利用野生型和突变型启动子转移RNA研究大肠杆菌与人启动子转移RNA以及相应的蛋白质合成启动因子IF2和eIF2之间的特异性相互作用的分子机制。Khorana实验室将研究脊椎动物光感受器视紫红质光激活后视觉过程(敏化和脱敏)中蛋白质-蛋白质相互作用的结合和动力学常数。最初的焦点将集中在激活的受体和G蛋白、转导蛋白之间的相互作用，以及受体和视紫红质激酶之间的相互作用。Kim实验室将研究HIV gp41核心的多肽，这些多肽被认为是HIV感染的抑制剂。将对这些多肽及其衍生物的结合和解离动力学进行研究，以便为开发治疗艾滋病的药物提供信息。Krieger实验室将研究细胞表面清道夫受体，这种受体结合化学修饰的脂蛋白，参与动脉粥样硬化的形成，识别和清除凋亡细胞以及有毒和致病物质，以及高密度脂蛋白代谢。Biacore仪器将用于探索这些不同的多配体受体的复杂结合特性。Sauer实验室致力于研究(I)抑制物-DNA相互作用，(Ii)细胞内蛋白酶如何识别目标为破坏的蛋白质中的特定序列，以及(Iii)影响Arc抑制物及其变体的同源和异源二聚化的因素。

The application by five P.I.'s proposes to use surface plasmon resonance for the kinetic and thermodynamic analysis of specific protein-protein and protein-nucleic acid interactions in a variety of biological systems. The RAJBHANDARY laboratory will perform studies of protein synthesis initiation in eubacteria and in eukaryotes. The molecular mechanisms underlying the specific interaction between the E. coli and human initiator transfer RNAs and the corresponding protein synthesis initiation factors IF2 and eIF2 will be studied using wild type and mutant initiator transfer RNAs. The KHORANA laboratory will study binding and kinetic constants of protein-protein interactions in visual processes (sensitization and desensitization) following light-activation of the vertebrate photoreceptor rhodopsin. Initial focus will be on interactions between activated receptor and the G protein, transducin, on the one hand and between the receptor and rhodopsin kinase on the other. The KIM laboratory will study peptides from the core of HIV gp41, that are known to be inhibitors of HIV infection. The kinetics of association and dissociation of these peptides, and derivatives of these peptides, will be investigated in order to provide information to assist the development of drugs to treat AIDS. The KRIEGER laboratory will study cell surface scavenger receptors which bind chemically modified lipoproteins and have been implicated in atherogenesis, in the recognition and clearance of apoptotic cells and toxic and pathogenic substances, and in HDL metabolism. BIACORE instrument will be used to explore the complex binding properties of these diverse, multiligand receptors. The SAUER laboratory is engaged in studies of (i) repressor- DNA interactions, (ii) how intracellular proteases recognize specific sequences in proteins targeted for destruction and (iii) factors that influence homo versus heterodimerization of the Arc repressor and its variants.