MULTIUSER EPR SPECTROMETER AT UCSD

这是加州大学圣地亚哥分校修改后的申请，要求提供资金购买先进的电子顺磁共振(EPR)光谱仪。加州大学圣迭戈分校成立了一个用户小组，由来自4个加州大学校园部门的8名教职员工和斯克里普斯研究所的1名工作人员组成；有8名NIH资助的PI代表。购买光谱仪将支持的个人研究活动包括研究血红素蛋白质和呼吸和光合作用蛋白质中的金属结合部位，金属蛋白簇和金属醌络合物的模型，受复杂调控信号影响的酶的构象变化，分子聚集体在脂双层上或膜内的组装，脂类与膜蛋白的相互作用，以及绿色荧光蛋白到发出红光的形式的光化学转化。选择的光谱仪是Bruker ELEXsys系统，工作在X波段频率。选择该仪器的理由包括其作为连续波谱仪的最先进能力，以及其容量将被扩大(未来将获得额外资金)，以进行更先进的EPR实验，如多频率EPR、Endor和ESEEM。我们预计未来会有这样的实验需要。还要求提供资金，用于购买适当的低温设备、用于更灵敏地记录蛋白质结合的自旋标记的TM110模腔和用于获得准确g值的核磁共振高斯计。该系统的总成本为390,571美元。提出了一项管理安排和一份长期运营计划。

This is a revised request from the University of California at San Diego for funds to purchase an advanced electron paramagnetic resonance (EPR) spectrometer. A User's Group has been established at UCSD involving 8 faculty and staff from 4 UCSD campus departments and 1 staff at The Scripps Research Institute; 8 NIH funded PI's are represented. The individual research activities to be supported by the purchase of the spectrometer include studies of metal binding sites in heme proteins and proteins of respiration and photosynthesis, models for metalloprotein clusters and metal quinone complexes, conformational changes in enzymes subject to complex regulatory signals, assembly of molecular aggregates on or within lipid bilayers, interactions of lipids with membrane proteins and the photochemical conversion of Green Fluroescent Protein to a form that emits red light. The spectrometer of choice is the Bruker ELEXSYS system, operating at X-band frequency. Justifications for the choice of this instrument include its state-of-the-art capabilities as a continuous wave spectrometer and its capacity to be expanded (with future additional funding) to carry out more advanced EPR experiments, such as multifrequency EPR, ENDOR and ESEEM. We anticipate a future need for such experiments. Funds are also requested for the purchase of appropriate cryogenic apparatus, a TM110 mode cavity for more sensitive recording of protein bound spin-labels and an NMR gaussmeter to obtain accurate g-values. The total cost of the system is 390,571 dollars. An Administrative Arrangement and a Long-Term Operation Plan are presented.