CORE--DNA SEQUENCING AND SYNTHESIS

DNA测序和合成核心将提供仪器， 自动化DNA使用方面的一般资源和技术专长 DNA测序及相关分子生物学技术在分析中的应用 基因结构和多态。我们将特别强调 利用这一信息来描绘易感性的遗传学 皮肤病。Core在自动测序方面拥有特殊的技能 杂合DNA模板直接定义等位基因，在计算机中- 辅助序列分析。它的两个DNA测序仪已经被改装成 执行自动微卫星多态分析以及 常规的自动测序。DNA测序与合成核心 将与分子诊断和突变协同工作 检测核心是确定微卫星的多态。这个 DNA测序和合成核心-也将作为一项技术 关于信息和培训的资源中心。核心包含 合成和提纯合成的资源和设备 为国家发改委调查人员和所有感兴趣的实验室提供的寡核苷酸 在皮肤的研究中。这一核心设施的两个主要特征是其 一系列全面的服务，旨在支持双方的需求 国家发改委拟议和未来的排序活动及其目标 在其专业领域对年轻的调查人员进行培训。

The DNA Sequencing and Synthesis Core will provide instrumentation, general resources and technical expertise in the use of automated DNA sequencing and related molecular biologic techniques for the analysis of gene structure and polymorphisms. Special emphasis will be placed on the use of this information to delineate the genetics of susceptibility to skin diseases. The Core has particular skill in automated sequencing of heterozygous DNA templates to directly define alleles and in computer- assisted sequence analysis. Its two DNA sequencers have been modified to perform automated microsatellite polymorphism analyses as well as conventional automated sequencing. The DNA Sequencing and Synthesis Core will work in concert with the Molecular Diagnostics and Mutation Detection Core on the determination of microsatellite polymorphisms. The DNA Sequencing and Synthesis Core will-also serve as a technical resource center regarding information and training. The Core contains resources and equipment for the synthesis and purification of synthetic oligonucleotides for SDRC investigators and all laboratories interested in the study of skin. Two key features of this core facility are its comprehensive range of services designed to support the needs of both proposed and future SDRC sequencing activities and its objective of training young investigators in the area of its expertise.