PILOT & FEASIBILITY STUDIES

危重病人常出现甲状腺功能减退。 对预后有重要意义。然而，目前还不清楚 甲状腺功能减退是器官功能障碍的原因之一，或者只是 EPI-急性脓毒症的现象。这项研究的目的是 测定外源性甲状腺激素改善肝脏功能的能力 败血症大鼠模型中的生物能量学。生物能量功能将是 通过测量肝脏中的高能磷酸盐水平进行评估 使用31P核磁共振波谱。研究中的实验动物 将在脓毒症后立即接受甲状腺治疗 归纳法。高能磷酸盐水平的比较 治疗组和非治疗组将展示其影响 甲状腺功能对肝脏生物能量学的影响初步数据显示 受伤但未受挑战的肝脏表现出明显的稳定 用三磷酸腺苷和无机磷(PI)评价生物能量功能 浓度。随后，当肝脏受到 代谢挑战(胰高血糖素)，PI/ATP增加 比率这一比率的增加表明了 从能量学的角度来看，肝脏功能失调。我们的 初步数据(没有高血糖素挑战)还没有 证明了败血症动物的肝脏具有生物能量 不稳定~因此，我们用胰升糖素对肝脏进行了测试。 这些数据的初步结果表明，肝脏 败血症动物的生物能量衰竭，而非败血症动物则如此 不。因此，我们现在建议研究 三碘甲腺原氨酸(T3)替代治疗对肝脏的影响 功能障碍。：

Decreased thyroid function often occurs in critically ill patients and is of prognostic significance. However, it is unknown if hypothyroidism is a cause of organ dysfunction or just an epi-phenomena of acute sepsis. The objective of this study is to determine the ability of exogenous thyroid hormone to improve hepatic bioenergetics in a septic rat model. Bioenergetic function will be assessed by measuring the high energy phosphate levels in the liver using 31P NMR spectroscopy. The experimental animals in the study will be given thyroid treatment immediately following septic induction. The comparison of the high energy phosphate levels between the treatment and non-treatment groups will demonstrate the influence of thyroid function on hepatic bioenergetics Preliminary data show that an injured but unchallenged liver exhibits apparently stable bioenergetic function as assessed by ATP and inorganic phosphate (Pi) concentrations. Subsequently, when the liver is subjected to a metabolic challenge (glucagon), there is an increase in the Pi/ATP ratio. This ratio increase demonstrates the severity to which the liver is dysfunctional from an energetics perspective. Our preliminary data (without the glucagon challenge) have not demonstrated the livers of the septic animals to be bioenergetically unstabIe~therefore, we tested the liver with a glucagon challenge. The preliminary results from these data demonstrate hepatic bioenergetic failure in septic animals, whereas non-septic animals do not. Accordingly, we now propose to study the effects of triiodothyronine (T3) replacement on modifying this hepatic dysfunction. :