MOLEC MODEL OF COFILIN REGULATION IN ACTIN FILAMENT DYNAMICS & CELL FUNCTIONS

脊椎动物视网膜的视觉是由光激活的G 位于光感受器盘膜上的蛋白质偶联途径 细胞。通过这一途径发出的信号涉及形成和 激活多种膜依赖蛋白复合体。我们 正在研究这些复合体的结构，因为它们存在于 通过电子结晶学对膜表面进行表征。我们已经组成了 异三聚体形式的二维晶体和螺旋阵列 并用冷冻电子显微镜对其进行成像。图像 对螺旋结构的处理揭示了 计算傅里叶变换到超过18，并对这些内容进行索引 转换正在进行中。

Vision in vertebrate retinas is initiated by a light-activated G protein coupled pathway located on disc membranes in the photoreceptor cells. Signaling through this pathway involves the formation and activation of a variety of membrane-dependent protein complexes. We are studying the structures of these complexes as they exist on membrane surfaces through electron crystallography. We have formed two-dimensional crystals and helical arrays of the heterotrimeric form of the G protein and imaged them by cryo-electron microscopy. Image processing of the helical structures has revealed layer lines in the computed Fourier transform to beyond 18
, and indexing of these transforms is in progress.