ELECTRON SPIN ECHO MODULATION OF DEOXYCO MYOGLOBIN & DEOXYCO PORPHYRIN MODELS

电子自旋回波调制光谱记录的蓝色 铜蛋白质rusticyanin和突变体缺乏这两个之一 组氨酸配体铜（H85A）。 W.T.和H85A形式的 蛋白质进行了深入研究，以充分表征ESEEM 铜配体中咪唑氮的光谱性质 领域 在三个脉冲中使用定时变量（tau选择） 实验作为一种手段，以确定峰已经建立。 一 识别峰和定量超超细粉的图解法 参数从实验数据也已证明。 的 ESEEM谱表明，两个自旋流形的m=1跃迁可能 通过在三脉冲实验中对τ的明智选择来观察。 这大大提高了模拟过程的准确性， 消除了由于不适当的关联而错误识别峰时的模糊性。 峰值的图形表示允许人们预测 “精确取消”的条件，从而消除 量化超超精细参数。 此外，缺乏 第二个组氨酸配体将更多的自旋密度放在剩余的组氨酸配体上， 组氨酸配体与铜。 论文已在此基础上发表

Electron spin echo modulation spectra were recorded for the blue copper protein rusticyanin and a mutant lacking one of the two histidine ligands to copper (H85A). The w.t. and H85A forms of the protein were examined in depth to fully characterize the ESEEM spectroscopic properties of imidazole nitrogens in the copper ligand field. The use of timing variables (tau selection) in the three pulse experiment as a means to identify peaks has been established. A graphical method of identifying peaks and quantifying superhyperfine parameters from experimental data has also been demonstrated. The ESEEM spectra suggest that m=1 transitions of both spin manifolds may be observed by a judicious choice of tau in a three pulse experiment. This greatly improves the accuracy of the simulation process and removes ambiguity in misidentifying peaks by an improper association. A graphical representation of the peaks allows one to predict the condition of "exact cancellation" and thereby remove ambiguities in quantifying the superhyperfine parameters. Further, the absence of the second histidine ligand puts more spin density onto the remaining histidine ligand to copper. Paper has been published based on this