CERAMIDE METABOLISM IN EPIDERMAL DIFFERENTIATION AND BARRIER FUNCTION

在表皮中鞘脂集中在上部， 分化的细胞层。 虽然有大量证据表明， 鞘脂在提供渗透性中的关键作用 屏障，对鞘脂生物合成的调节知之甚少 在表皮中。 培养的人角质形成细胞应该提供有用的 研究鞘脂生物合成与表皮细胞之间联系的模型 分化，因为这些培养物，当生长在空气培养基中时， 界面，显示高级形态分化，以及 与平行组相比，鞘脂含量和生物合成增加 沉浸文化 在本提案中，我们将测试以下内容： 1. 角质形成细胞的鞘脂组成和合成变化 与其分化程度有关。 2. 鞘脂代谢产物本身可调节 表皮分化

In the epidermis sphingolipids are concentrated in the upper, differentiated cell layers. Although substantial evidence supports a critical role for sphingolipids in the providion of the permeability barrier, little is known about the regulation of spingolipid biosynthesis in the epidermis. Cultured human keratinocytes should provide a useful model to study the link between spingolipid biosynthesis and epidermal differentiation because these cultures, when grown at an air-medium interface, display advanced morphologic differentiation, as well as increased spingolipid content and biosynthesis in comparison to parallel immersed cultures. In this proposal we will test the following: 1. Sphingolipid composition and synthesis by keratinocytes varies in relation to their degree of differentiation. 2. Products of sphingolipid metabolism may themselves regulate epidermal differentiation.