CORE--MOLECULAR BIOLOGY

CWRU的分子生物学核心设施已经运行了近8年 多年来，为研究提供最先进的科学能力 社区，特别是在昂贵的仪器设备或独特的技术 功能是必需的。为此目的，核心基金已获得 各种仪器，其核心是2个DNA合成器和 一种蛋白质微测序仪。根据CWRU内的当前限制 社区，提议扩大核心设施，以提供 以下内容：多肽合成、DNA测序和大规模(最高可达 20升)哺乳动物和微生物培养物的发酵。这个 这些能力研究项目的必要性以及新的 调查人员在过去两年增加了CWRU的教职员工。基金是 要求购买(一)，一台DNA测序仪(二)，一台大型 用于微生物发酵的发酵罐和离心机；(Iii)哺乳动物 发酵设备。此外，还要求提供资金，以取代一个 八年前的DNA合成器。这一替代将允许更大的 待制备的寡核苷酸数量，并将大大升级两者 DNA寡核苷酸合成的可靠性和能力 有或没有非标准核苷酸。除了……之外 工具，请拨资金用于部分支助三个 研究技术人员，与DNA测序、多肽合成相关 (核心设施中有一个多肽合成器，可用于 然而，CWRU社区过去的需求不足以 需要为这项工作聘请技术人员)和生物发酵。这些 个人将只与一个人的独特需求相关联 一种既涉及修复功能又涉及分析功能的工具。 技术人员的工资支持将在整个年内逐渐减少 资助期，并将按使用费收回。用户的计算 费用反映了用品、工资支助和年度维护的费用 合同(由于仪器内部复杂的电子设备 核心设施，所有设施都由延长的维护定期覆盖 与制造商的协议，尽管在某些情况下 当出现问题时，适当的个人可能会进行故障排除)。 核心基金在过去八年中一直在运作，目前 熟悉用户资费的建立。这是一种惯例 仅在与以下情况相关联的情况下才能收回费用 每个服务并且不具有由另一个服务支持的任何服务。

The Molecular Biology Core Facility at CWRU has operated for almost 8 years, providing state-of-the-art scientific capabilities to the research community, especially where expensive instrumentation or unique technical capabilities are required. To this end, the Core Facility has acquired a variety of instruments, the heart of which is 2 DNA synthesizers and a protein microsequencer. Based upon current limitations within the CWRU community, it is proposed that the Core Facility be expanded to provide the following: peptide synthesis, DNA sequencing, and large scale (up to 20 liters) fermentation of mammalian and microbial cultures. The necessity for these capabilities research projects as well as new investigators added to the CWRU faculty in the last two years. Funds are requested for the purchase of (i), a DNA sequencer (ii), a large scale fermenter and centrifuge for microbial fermentation and (iii), mammalian fermentation equipment. In addition, funds are requested to replace an eight year old DNA synthesizer. This replacement will allow for larger numbers of oligonucleotides to be prepared and will greatly upgrade both the reliability and capability of the synthesis of DNA oligonucleotides with or without non-standard nucleotides. In addition to instrumentation, funds are requested for partial support of three research technicians, associated with DNA sequencing, peptide synthesis (a peptide synthesizer exists in the Core Facility and is available to the CWRU community, however, past demand had not been sufficient to warrant hiring a technician for this effort) and bio-fermentation. These individuals will be associated with the unique demands of just a single instrument which will involve both reparative and analytical functions. Salary support of technical staff will gradually decrease throughout the funding period, and will be recovered by user fee. Calculation of user fees reflects the cost of supplies, salary support and annual maintenance contracts (due to the complex electronics of the instrument within the Core Facility, all are routinely covered by extended maintenance agreements with the manufacturer, although in some instances the appropriate individual may do trouble-shooting when a problem arises). The Core Facility has been operational for the last eight years and is familiar with the establishment of users fees. It has been the practice of the Core Facility to recover costs only as they are associated with each service and to not have any service supported by another.