DOUBLE-STRANDED RNA AND THE INTERFERON SYSTEM

本项目的总体目标是了解 双链RNA的转录信号转导。的表达方式 几种干扰素诱导基因是由双链直接诱导的 核糖核酸。我们将研究双链RNA是如何调节 基因转录。为此，顺式作用序列 所需的双链RNA将被识别和 调节这一诱导的同源反式作用因素将是 分析过了。该项目的另一个目标是识别细胞蛋白质。 它能特异性地结合双链RNA。这些蛋白质将是 纯化后，将分离它们的cDNA克隆，并推测它们的作用 我们将评估双链RNA对转录信号的影响。 拟议的研究将使人们更好地了解 双链RNA诱导基因的表达调控 编码许多重要的调节蛋白，如干扰素， 白介素类和细胞黏附分子。我们的研究还揭示了 所使用的信号转导通路之间的重叠性质 干扰素和双链RNA诱导转录 相同的基因。

The overall goal of this project is to understand the mechanisms of transcriptional signaling by double-stranded RNA. the expression of several interferon-inducible genes is directly induced by double-stranded RNA. We will investigate how double-stranded RNA regulates the transcription of genes. For this purpose, the cis-acting sequences required for the effects of double-stranded RNA will be identified and the cognate trans-acting factors which mediate this induction will be analyzed. Another aim of the project is to identify cellular proteins which specifically bind double-stranded RNA. These proteins will be purified, their cDNA clones will be isolated, and their putative roles is transcriptional signaling by double-stranded RNA will be evaluated. the proposed studies will provide a better understanding of the regulation of expression of double-stranded RNA-inducible genes which encode many important regulatory proteins such as interferons, interleukins, and cell adhesion molecules. Our studies also reveal the nature of overlap between the signal transduction pathways used by interferons and double-stranded RNA for inducing transcription of the same genes.