PILOT--IDENTIFICATION OF COPY NUMBER ALTERATIONS IN ORAL CANCER

这项研究建议使用一种新的细胞遗传学方法--比较基因组学 杂交(CGH)，以鉴定口腔中新的基因改变 空洞鳞状细胞瘤。在比较基因组杂交中， 来自肿瘤的染色体DNA被统一地用绿色标记 荧光色素和正常DNA用红色荧光色素标记。这个 然后，标记的DNA同时与正常人杂交 中期展开。绿色与红色荧光比的定量测定 沿着每条染色体的长度允许区域增加或 减少了要识别和映射的副本数量 做实验。该方法已成功地应用于小说的识别 小到几个的非整倍体、扩增和缺失 兆级数据库。 50例口腔肿瘤标本按顺序行CGH检查 以确定所有可能导致细胞遗传学改变 这种肿瘤类型中的肿瘤发生。初步数据表明，CGH可以 成功地对从固定、石蜡包埋的DNA中提取的DNA进行了操作 肿瘤组织和来自各种肿瘤的新鲜肿瘤标本 包括口腔肿瘤和肺部肿瘤。由以下人员标识的更改 CGH将与放射治疗的疗效相关，定义为 局部控制肿瘤生长的效率。此外，小说 改变基因可能成为未来位置克隆的候选基因 为确定该基因的分子功能所做的努力(S) 牵涉其中。

This study proposes to use a new cytogenetic method, comparative genomic hybridization (CGH), to identify novel genetic alterations in oral cavity squamous cell tumors. In comparative genomic hybridization, chromosomal DNA from tumors is uniformly labelled with a green fluorochrome and normal DNA is labelled with a red fluorochrome. The labelled DNAs are then simultaneously hybridized to normal human metaphase spreads. Quantitation of the green to red fluorescence ratio along the length of each chromosome allows regions of increased or decreased copy number to be identified and mapped in a single experiment. The method has been used successfully to identify novel aneuploidies, amplifications and deletions as small as several megabases. Fifty specimens of oral cavity tumors will be examined by CGH in order to identify all cytogenetic alterations that may contribute to tumorigenesis in this tumor type. Preliminary data indicate that CGH can be successfully performed on DNA extracted from fixed, paraffin-embedded tumor tissue and from fresh tumor specimens from a variety of tumors including oral cavity tumors and lung tumors. Alterations identified by CGH will be correlated with efficacy of radiotherapy as defined by efficiency of local control of tumor growth. In addition, novel alterations could become candidates for future positional cloning efforts designed to determine the molecular function of the gene(s) involved.