GALACTOSYLTRANSFERASE BIOSYNTHESIS AND MAMMARY GLAND TUMORS

切除未经治疗的乳腺组织、哺乳期乳腺组织和 异种生物(二甲基苯并[a]菲和硫丹)诱导雌性大鼠 将被用来进行分离和生化鉴定 可能的细胞(质)膜相关半乳糖基转移酶(S) 作为细胞间接触和接触抑制的调节分子； 后者被怀疑是肿瘤发生的原因。这些乳腺 肿瘤组织将接受亚细胞分割， 电子显微镜、亲和层析、SDS-PAGE、高压 比较毛细管气相色谱、薄层层析 原位酶(S)部分分析。这些研究试图确定 膜相关蛋白可能的功能作用(S) 半乳糖基转移酶(S)与细胞增殖。

Excised mammary gland tissues from non-treated, lactating and tumors from Xenobiotic (dimethylbenz[a]anthracene and endosulfan) induced female rats will be utilized to conduct isolation and biochemical characterization of putative cell (plasma) membrane associated galactosyltransferase(s) as regulation molecules in cell-cell contact and contact inhibition; the latter suspected of being causal in tumorigenesis. These mammary gland and tumor tissues will be subjected to subcellular fractionation, electron microscopy, affinity chromatography, SDS-PAGE, High Pressure Liquid Chromatography, and Thin-Layer Chromatography for comparative IN SITU enzyme(s) moiety analyses. These studies seek to determine the putative functional role(s) of membrane-associated galactosyltransferase(s) in cell proliferation.