HIGH PRESSURE DIFFERENTIAL SCANNING CALORIMETER

压力提供了一个额外的热力学变量 大分子稳定性的研究。压力微扰将 取代大分子的构象平衡 到每个构象的相对体积。压力是不可能的 只影响蛋白质的稳定性而不引入 化学变量，但也能部分稳定选定 折叠构象。因此，它应该成为一个重要的 研究蛋白质稳定性和折叠机制的仪器。 一种具有操作能力的差示扫描量热仪 在不同的恒定压力下代表了长期的设计计划 生物热量测量中心。在本年度内，最初 启动了可行性研究。可想而知，这是 发展将分两个阶段进行。在第一阶段中，DSC具有 能够支持高达~500个ATM的加固信元将是 发展起来的。在第二阶段，一个热量计，其中内部 以及电池的外部都会被加压 都是设计好的。这第二台仪器将能够维持 压力接近4,000大气压

Pressure provides an additional thermodynamic variable for the study of macromolecular stability. A pressure perturbation will displace the conformational equilibrium of a macromolecule according to the relative volume of each conformation. Pressure is capable not only of affecting the stability of a protein without introducing a chemical variable, but is also able to stabilize selected partially folded conformations. As such, it should become an important instrument for the study of protein stability and folding mechanisms. A differential scanning calorimeter with the capability of operating at different constant pressures represent a long term design plan for the Biocalorimetry Center. During the current year, initial feasibility studies were initiated. It is visualized that this development will be made in two stages. In the first stage a DSC with reinforced cell capable of sustaining up to ~500 atm will be developed. In the second phase, a calorimeter in which the interior as well as the exterior of the cell are pressurized will be designed. This second instrument will be capable of sustaining pressures approaching 4,000 atm