CORE--VIROLOGY FACILITY

病毒学核心病毒学核心的目的是为小鼠体内的病毒感染提供设施和技术援助。生物安全三级(BL3)生物遏制套间内的一个房间将被分配用于该项目的工作。一名研究技术人员将负责给小鼠接种病毒、随后的监测和监测，以及小鼠的出血和组织的采集，然后可能会分发给其他项目实验室或形态核心实验室。该病毒学核心还将负责病毒的生长、制备、纯化和检测，抗病毒抗体的产生，以及LCMV特异性T细胞转基因小鼠的维持。它将制备载肽的Ig G-MHC二聚体来定量病毒特异性T细胞，并将进行一些抗病毒CTL试验、T细胞增殖试验、细胞因子产生细胞试验和有限稀释试验，以监测T细胞的频率、特异性和功能。

VIROLOGY CORE The purpose of the Virology Core is to provide facilities and technical assistance for the conduction of virus infections in mice. A room within a biosafety level three (BL3) Biocontainment Suite will be assigned for work on this project. A research technician will be responsible for the inoculation of mice with viruses, for their subsequent surveillance and monitoring, and for the bleeding of mice and harvesting of tissues, which may then be distributed to the other Project Laboratories or to the Morphology Core Lab. This Virology Core will also be responsible for the growth, preparation, purification, and assay of viruses, for the generation of antiviral antibodies, and for the maintenance of LCMV- specific T cell transgenic mice. It will prepare peptide-loaded IgG-MHC dimers to quantify virus-specific T cells, and it will perform some of the antiviral CTL assays, T cell proliferation assays, cytokine-producing cell assays, and limiting dilution assays to monitor T cell frequency, specificity, and function.