Core--Gene expression vector

基因表达载体核心(GEVC)将为内森休克中心的附属研究人员提供最先进的分子生物学资源，帮助他们研究正常衰老和与年龄相关的疾病的病理生理学。GEVC将协助开发和生产以单纯疱疹病毒为基础的扩增载体为基础的基因转移试剂。这些都适合于体外和体内的基因转移研究。此外，GEVC还将使用“实时”聚合酶链式反应进行核酸定量测量。虽然扩增子载体已经被证明是一种非常有效的载体，可以将遗传物质输送到几个动物物种的许多组织和细胞类型，但它对老龄社区的研究人员的可及性将促进现有的研究，并使新的研究成为可能。同样，“实时”定量聚合酶链式反应技术已成为从细胞和组织中定量特定核酸序列(RNA或DNA)的一种可重复性和高通量的方法。然而，由于与初始设备成本相关的巨额费用，大多数调查人员无法利用这项宝贵的技术。通过利用罗切斯特大学现有的专业知识和设施，GEVC寻求为休克中心的地方、地区和国家研究人员以及其他研究与衰老相关问题的研究人员提供基于扩增载体的基因转移和定量聚合酶链式反应技术。GEVC将设在老龄化和发育生物学中心内新建的Arthur Kornberg医学研究大楼的一楼。GEVC将由William J.Bowers博士指导和Howard J.Federoff博士共同指导，而日常核心活动将由一名实验室技术助理执行。全球环境研究中心科学监督委员会将由联合主任和John Olschowka博士(定量聚合酶链式反应顾问)组成。

The Gene Expression Vector Core (GEVC) will provide Nathan Shock Center-affiliated investigators with state-of-the-art molecular biological resources that facilitate their studies of normal aging and pathophysiology of age-related disease. The GEVC will assist in the development and production of gene transfer reagents based on the herpes simplex virus- based amplicon vectors. These are suitable for in vitro and in vivo gene transfer studies. In addition the GEVC will perform quantitative nucleic acid measurements using "real-time" PCR. While the amplicon vector has been shown to be a very effective vehicle for the delivery of genetic material to numerous tissue and cell types of several animal species, its accessibility to investigators in the aging community will facilitate existing studies and enable new ones. Similarly, the "real-time" quantitative PCR technology has provided to be a reproducible and high throughput method for quantitation of specific nucleic acid sequences (RNA or DNA) from cells and tissues. However, due to the large expense related to initial equipment costs, most investigators are unable to utilize this valuable technology. By taking advantage of the expertise and facilities that already exists at the University of Rochester, the GEVC seeks to provide both amplicon vector-based gene transfer and quantitative PCR technologies to Shock Center investigators locally, regionally and nationally and other researchers pursuing aging-related problems. The GEVC will be housed on the first floor of the newly constructed Arthur Kornberg medical Research Building within the Center for Aging and Developmental Biology. The GEVC will be directed by Dr. William J. Bowers and co-directed by Dr. Howard J. Federoff and, while day-to-day core activities will be performed by a laboratory technical associate. The GEVC scientific oversight committee will consist of the co-directors and Dr. John Olschowka (quantitative PCR consultant).