CORE--MOLECULAR IMMUNOLOGY

核心I：分子免疫学 免疫学的核心计划最初被设计为作为一个 为希望进行研究研究人员提供知识和技术资源 艾滋病相关问题的免疫学方面。这就需要招聘 发展中央湿实验室和流式细胞术 既能处理受病毒感染的材料，又能提供 艾滋病相关研究所需的独特资源和试剂。 这些目标已经通过构建一个集中的CFAR来实现 免疫学实验室配备细胞和分子研究毗邻 CFAR BL-3实验室，为流式细胞术实验室配备 封闭的流动系统，并确定一组核心调查员 致力于建立艾滋病免疫学项目。集中式CFAR 实验室一直处于方法开发的最前沿， HIV和SIV细胞免疫分析试剂。的 由核心人员产生的试剂如纯化的病毒蛋白， 重组牛痘病毒和逆转录病毒穿梭载体，表型 靶细胞和单克隆抗体可用于中心 科学家个人使用或合作研究。艾滋病的传播 细胞仪设施，这是我们机构唯一的设施， 处理活的HIV感染细胞，广泛用于艾滋病相关研究 来自多个学科的研究人员。 该核心支助的主要项目包括以下研究： HIV疾病的免疫发病机制，无论是在新感染的人， 灵长类动物模型;（B）人类和灵长类动物模型中的疫苗开发;（c） 粘膜免疫;（d）用遗传学方法治疗HIV的特异性T细胞疗法， 修饰的T克隆;和（e）免疫生物学和机会性免疫缺陷的治疗。 在HIV感染的宿主中发展的感染。免疫学核心提出 继续提供：（a）技术援助、培训和试剂 分析对HIV和SIV的免疫应答所必需的;和（B） 技术援助、试剂和设备， 复杂的流式细胞术分析和病毒感染的分离， 未感染的细胞为了满足参与调查的人员的需要， 这些项目，免疫学核心建议扩大其努力， 包括：（a）肽合成设施，用于T HIV/SIV中的细胞和抗体表位;放置升级的细胞 专用BL-3实验室内的分析仪/分选机;以及（c）体液免疫分析仪， 能够量化抗体应答免疫设施， 灵长类动物标本的中和活性。

CORE I: Molecular Immunology The core program in immunology was originally designed to function as an intellectual and technical resource for investigators wishing to study immunologic aspects of AIDS-related issues. This required the recruitment of scientists and development of central wet laboratory and flow cytometry facilities capable both of handling virus-infected material and providing the unique resources and reagents necessary for AIDS-related research. These goals have been accomplished by constructing a centralized CFAR immunology laboratory equipped for cellular and molecular studies adjacent to the CFAR BL-3 laboratory, equipping a flow cytometry laboratory with a closed flow system, and identifying a group of core investigators committed to establishing an AIDS immunology program. The centralized CFAR laboratory has been at the forefront of the development of methods and reagents for the analysis of cellular immunity to HIV and SIV. The reagents generated by core staff such as purified viral proteins, recombinant vaccinia virus and retrovirus shuttle vectors, phenotyped target cells, and monoclonal antibodies, are available to center scientists for individual use or collaborative studies. The AIDS flow cytometry facility, which is the only facility at our institution that can handle viable HIV-infected cells, is widely used for AIDS-related studies by investigators from many disciplines. The major projects supported by the core include studies of: (a) immunopathogenesis of HIV disease, both in newly-infected humans and in primate models; (b) vaccine development in humans and primate models; (c) mucosal immunity; (d) specific T cell therapy of HIV with genetically- modified T clones; and (e) immunobiology and therapy of opportunistic infections developing in HIV-infected hosts. The immunology core proposes to continue to provide: (a) technical assistance, training, and reagents necessary for the analysis of immune responses to HIV and SIV; and (b) technical assistance, reagents and the equipment necessary for sophisticated flow cytometric analysis and isolation of virus-infected and uninfected cells. In response to the needs of investigators involved in these projects, the Immunology core proposes to expand its efforts to include: (a) a peptide synthesis facility for production and mapping of T cell and antibody epitopes in HIV/SIV; placement of an upgraded cell analyzer/sorter inside a dedicated BL-3 laboratory; and (c) a humoral immunity facility capable of quantifying antibody responses and neutralizing activity in specimens from primates.