CORE--VIRAL PATHOGENESIS

病毒致病核心作为分离株的集中来源 以及与HIV-1、HIV-2和SIV相关的检测。此外，核心还充当 用户和UCSD CFAR成员实验室之间的接口 专门研究影响艾滋病毒感染者的疱疹病毒(CMV， HSV和HHV-8)。提供四种基本类型的服务：(1)作为病毒 资源，核心从广泛的集合中提供等分 HIV-1、HIV-2和SIV病毒株的特征和滴度。(2)作为 作为一种细胞资源，核心提供病毒敏感细胞，包括细胞 分离的CD4+T细胞和巨噬细胞。(3)作为一种病毒 表征资源，核心提供细胞向向性测试， 合胞体诱导(SI)与非SI、共受体使用、细胞病变 与非细胞病变相比，复制动力学、药物敏感性和 HIV的其他特性。(4)作为一种筛选服务，核心提供 研究疗法的初步测试(抗病毒化合物， 基因疗法、抗体中和等)。这些资源使它成为 无论是新手还是有经验的调查人员都更容易追查他们的 病毒学研究思路。如果没有核心，每个调查员都需要 建立和验证其所需的不同方法和分析 工作，这可能需要几个月的时间。此外，Core的许多用户没有BL3 培训或体验，因此需要花费几个月的时间 努力获得使用这些病毒所需的许可。而当 核心并不意味着要取代由 作为主要项目之一的调查员，它是进行 中试实验和抗病毒活性化合物的筛选。

The Viral Pathogenesis Core serves as a centralized source for isolates and assays related to HIV-1, HIV-2, and SIV. In addition, the Core acts as an interface between users and the laboratories of UCSD CFAR members specializing in the herpes virus affecting HIV-infected individuals (CMV, HSV, and HHV-8). Four basic types of services are provided: (1) As a viral resource, the Core provides aliquots from a wide collection of characterized and titered viral strains of HIV-1, HIV-2, and SIV. (2) As a cell resource, the Core provides virus susceptible cells, including cell lines, isolated CD4+ T cells and macrophages. (3) As a virus characterization resource, the Core provides testing for cell tropisms, syncytium-inducing (SI) versus non-SI, co-receptor usage, cytopathic versus non-cytopathic, replication kinetics, drug susceptibilities, and other properties of HIV. (4) As a screening service, the Core provides the initial testing for investigational therapeutics (antiviral compounds, gene therapies, antibody neutralization, etc.). These resources make it easier for both novices and experienced investigators to pursue their virological research ideas. Without the Core, each investigator would need to set up and validate the different methods and assays required for their work, which could take months. Also, many of the Core's users have no BL3 training or experience themselves, and thus would need to expend months of effort on gaining the permission needed to work with these viruses. While the Core is not meant to replace a full scale research program set up by an investigator as one of the major projects, it is ideal for conducting pilot experiments and the screening of compounds for antiviral activity.