CORE--DNA SEQUENCING AND SYNTHESIS

DNA测序和合成核心将提供仪器， 使用自动DNA的一般资源和技术专长 测序和相关的分子生物学技术，用于分析 基因结构和多态性。将特别强调 使用这些信息来描述易感性的遗传学， 皮肤病核心在自动测序方面有特殊的技能， 杂合DNA模板直接定义等位基因和计算机- 辅助序列分析。它的两个DNA测序仪已经被修改， 进行自动化微卫星多态性分析，以及 常规自动测序。DNA测序和合成核心 将与分子诊断和突变 检测核心对微卫星多态性的测定。的 DNA测序和合成核心也将作为一个技术 信息和培训资源中心。所述芯含有 用于合成和纯化合成药物的资源和设备 SDRC研究人员和所有相关实验室的寡核苷酸 在皮肤的研究中。这一核心设施的两个主要特点是， 全面的服务，旨在支持双方的需求 拟议的和未来的SDRC排序活动及其目标， 在其专长领域培训年轻调查员。

The DNA Sequencing and Synthesis Core will provide instrumentation, general resources and technical expertise in the use of automated DNA sequencing and related molecular biologic techniques for the analysis of gene structure and polymorphisms. Special emphasis will be placed on the use of this information to delineate the genetics of susceptibility to skin diseases. The Core has particular skill in automated sequencing of heterozygous DNA templates to directly define alleles and in computer- assisted sequence analysis. Its two DNA sequencers have been modified to perform automated microsatellite polymorphism analyses as well as conventional automated sequencing. The DNA Sequencing and Synthesis Core will work in concert with the Molecular Diagnostics and Mutation Detection Core on the determination of microsatellite polymorphisms. The DNA Sequencing and Synthesis Core will-also serve as a technical resource center regarding information and training. The Core contains resources and equipment for the synthesis and purification of synthetic oligonucleotides for SDRC investigators and all laboratories interested in the study of skin. Two key features of this core facility are its comprehensive range of services designed to support the needs of both proposed and future SDRC sequencing activities and its objective of training young investigators in the area of its expertise.