DNA AND HYBRID STRUCTURE BY SOLUTION NMR

最近邻语境对局部碱基对影响的系统研究 结构将通过完成基于核磁共振的解决方案来继续 CGNatNCG系列中剩余的2个构造的结构和 CGNTANCG系列中剩下的三个结构。这5项建议 序列是Bam H1、Sph I、Nhe I、Eco Rv和 Kpn I限制性内切酶。 在B‘-DNA中形成B-DNA所需的临界大小的问题 环境将通过研究一系列的双重结构来阐明。 键入A5NnT5(其中n从1到4变化)以解释凝胶 寡聚N5-iAiTiN5-I序列中的延迟弯曲分析。这个 B-B‘结处和B’-B结处的折弯结构将为 研究了非对称十二烷基团和对称十六烷基团 以便更好地确定侧翼B-DNA片段的螺旋轴。 弯曲的结构和程度也将作为函数进行研究 侧翼上下文序列和G残基的结构效应 将对插入B‘ogo-A区的人进行调查。 DNA双链和杂交体之间的共价连接结构 DNA：RNA双链的核磁共振研究，特别是逆转录病毒连接 序列和右侧长末端重复序列的双链DNA序列 将对HIV-I进行调查，试图阐明其结构 这是这种LTR整合到人类基因组中的能力的基础。 除了这些目标之外，在特定序列上的协作工作 将进行其他计划实验室感兴趣的项目，以及 同位素固态(戏剧)原子距离测量将是 与Drobny实验室合作记录结构特征 在我们的高分辨率结构研究中观察到。

A systematic study of nearest neighbor context effects on local base pair structure will be continued by completing the NMR-based solution structures of the 2 remaining structures in the CGNATNCG series and the three remaining structures in the CGNTANCG series. These 5 proposed sequences are the restriction sites for Bam H1, Sph I, Nhe I, Eco RV and Kpn I restriction endonucleases. The question of the critical size required to nucleate B-DNA in a B'-DNA environment will be elucidated by studying a series of duplexes of the type A5NnT5 (where n is varied from 1-4) in order to interpret gel retardation bending assays in oligomerized N5-iAiTiN5-i sequences. The structure of the bend at B-B' junctions and B'-B junctions will be studied in non-symmetrical dodecamers and in symmetrical hexadecamers in order to better define the helical axis of the flanking B-DNA segments. The structure and extent of bending will also be studied as a function of the flanking context sequence and the structural effects of G residues inserted into the B'oligo-A tract will be investigated. The structure of covalent junctions between DNA duplexes and hybrid DNA:RNA duplexes will be studied by NMR, especially retroviral junction sequences, and the ds DNA sequence at the right long-terminal-repeat of HIV-I will be investigated in an attempt to elucidate the structural basis for the ability of this LTR to integrate into the human genome. In addition to these goals, collaborative work on particular sequences of interest to other program laboratories will be carried out, and isotopic solid-state (DRAMA) atomic distance measurements will be undertaken with the Drobny lab to document structural peculiarities observed in our high-resolution structural studies.