MOLECULAR ANALYSIS OF NEUTROPHIL ACTIVATION BY CHEMOATTRACTANTS

当这个项目开始时，它的目的是 确定调节吞噬细胞迁移的精确因素， 感染和炎症的部位，重点放在最初的 细胞与细胞外信号分子的相互作用 作为化学引诱物。吞噬细胞趋化因子的例子 包括细菌衍生的N-甲酰肽， 补体衍生肽C5 a，以及一大类结构上 相关的肽称为趋化因子。最近，该项目 已经扩展到包括淋巴细胞化学引诱物受体。 此外，我们发现各种微生物病原体， 包括艾滋病毒，利用化学引诱受体作为它们的一部分， 生命周期因此，我们的具体目标也扩大到 （一）确定不同的结构; 化学引诱物受体;（ii）确定化学物质 化学引诱物的激活和调节机制 受体;（iii）描绘信号转导途径 通过化学引诱物激活;（iv）确定一般和 每种化学引诱物受体的专门生物学作用;以及 (v)描述的生物作用和作用机制， 化学引诱物受体在微生物发病机制中的作用。

When this project originated, its purpose was to determine the precise factors regulating phagocyte migration to sites of infection and inflammation, focusing on the initial interaction of the cell with extracellular signaling molecules known as chemoattractants. Examples of phagocyte chemoattractants include bacterially-derived N-formyl peptides, the complement-derived peptide C5a, and a large class of structurally related peptides known as chemokines. More recently, this project has expanded to include lymphocyte chemoattractant receptors. Moreover, we have discovered that various microbial pathogens, including HIV, exploit chemoattractant receptors as part of their life cycle. Thus, our specific objectives have also expanded to include the following: (i) to determine the structure of distinct chemoattractant receptors; (ii) to determine the chemical mechanism of activation and regulation of chemoattractant receptors; (iii) to delineate the signal transduction pathways activated by chemoattractants; (iv) to determine the general and specialized biological roles of each chemoattractant receptor; and (v) to delineate the biological role and the mechanism of action of chemoattractant receptors in microbial pathogenesis.