CYTOSKELETON MEMBRANE INTERACTIONS AT CELL SUBSTRATE ADHESIONS

研究骨形成对于理解骨的形成和骨的形成具有重要价值。 骨质疏松症的发生机制和改善骨科固位力 植入物. 我们正在研究它们的运动，焦点接触， 破骨细胞的足状体。 细胞膜-细胞膜相互作用. 将结合FESEM分析细胞基质粘附 和能量过滤TEM技术。 我们的研究将包括 局灶性接触/粘连斑块的特征性发现于 成纤维细胞和神经生长锥。 此外，我们亦会研究 细胞-基质粘附的玫瑰花结样结构（podosomes） 在骨形成过程中， 再吸收 我们的观察将包括：I）FESEM成像的 用Lim和Ris的方法完整和Triton-lysed腹面 （1993），包括适当蛋白质的免疫金标记。 （二） Triton溶解后的背侧视图相同。 3)厚膜的场发射扫描电镜成像 提取后与基底成直角的细胞切片 epon（Ris and Malecki，1993）. 4)细胞粘连的成像 能量过滤透射电镜下的全标本和厚切片。

Studying bone formation is of great value for understanding the mechanisms of osteoporosis and improving the retention of orthopedic implants. We are studying the locomotion, focal contacts and podosomes of osteoclasts. Cytoskeleton-membrane interactions at cell-substrate adhesions will be analyzed using a combination of FESEM and Energy Filtering TEM techniques. We will include in our study focal contacts/adhesion plaques characteristically found in fibroblasts and nerve growth cones. In addition, we will also study the rosette-like structure of cell-substrate adhesions (podosomes) found in the thin peripheral lamellae of osteoclasts during bone resorption. Our observations will include: I)FESEM imaging of the intact and Triton-lysed ventral surface by the method of Lim and Ris (1993), including immunogold labeling of the appropriate proteins. 2) same of the dorsal view after Triton lysis. 3) FESEM imaging of thick sections of cells at right angle to the substrate after extraction of the epon (Ris and Malecki, 1993). 4) Imaging of cell adhesions in whole mounts and thick sections by Energy Filtering TEM.