TRANSVERSE TUBULAR STRUCTURE IN SKELETAL MUSCLE DEFECTS: MICE, CONTRACTION

我们正在研究横管系统的结构。 骨骼肌兴奋收缩偶联缺陷小鼠。 在骨骼肌和心肌中，血浆的电刺激 膜使L型钙通道开放。此事件会触发 进一步从细胞内贮藏物中释放钙。这种钙的释放导致 肌肉收缩。L型钙离子通道多见于 横管系统，一个连续的网状内陷 表面膜。我们希望能够绘制出 骨骼肌和心肌中的T管系统贯穿始终 发展。我们已经尝试使用单光子技术在 我们的实验室，对分辨率或深度不满意 我们获得的穿透力。我们将使用多光子成像技术 在IMR的设施，以便从我们的 标本。

We are studying the structure of the transverse tubular system in mice defective in excitation-contraction coupling in skeletal muscle. In skeletal and cardiac muscle, electrical excitation of the plasma membrane causes L-type Ca channels to open. This event triggers a further Ca release from intracellular stores. This Ca release causes muscle contraction. Most of the L-type Ca channels are found in the transverse tubular system, a continuous network invaginations of the surface membrane. We would like to be able to map out the pattern of the t-tubule system in skeletal and cardiac muscle throughout development. We have attempted this using single photon techniques in our laboratory and were not satisfied with the resolution or depth penetration that we obtained. We will use the multiphoton imaging facilities at the IMR in order to get more information out of our specimens.