LOCALIZATION OF NADH GLUTAMATE SYNTHASE IN ALFALFA ROOT NODULES

紫花苜蓿根瘤具有固定大气的能力 氮素与土生细菌根瘤菌的共生 梅里洛蒂。许多酶参与同化 共生固定氮。一种是依赖NADH的谷氨酸。 合成酶(NADH-GOGAT)，由谷氨酰胺和谷氨酸形成两种谷氨酸 甲氧基戊二酸。这种酶被认为是速度限制步骤。 这条路。为了了解氮的生物化学 在根瘤的同化过程中，NADH-GOGAT的定位至关重要。 我们会把根瘤的样本高压冷冻，然后再冷冻 替换、嵌入和分段。切片将进行免疫组织化学染色 为了定位NADH-GOGAT，在电子显微镜下进行了检查。

alfalfa root nodules have the capability to fix atmospheric dinitrogen through symbiosis with the soil born bacteria Rhizobium meliloti. Many enzymes are involved in the assimilation of symbiotically fixed nitrogen. One is the NADH-dependent Glutamate Synthase (NADH-GOGAT) which forms two Glutamate out of Glutamine and a-oxoglutarate. The enzyme is thought to be the rate limiting step in this pathway. In order to understand the biochemistry of the nitrogen assimilation in root nodules, localization of NADH-GOGAT is crucial. We will high-pressure freeze samples of root nodules and then freeze substitute, embed and sectioned. Sections will be immunostained and examined in the TEM in order to localize NADH-GOGAT.