CRYO HRSEM: BROME MOSAIC VIRUS DERIVED VIRUS LIKE PARTICLES FORMED IN YEAST

已发现类似病毒的颗粒在酵母中聚集 雀麦花叶病毒外壳蛋白在病毒复制中的表达 流程。低温透射电子显微镜和图像重建显示了 28 nm T=3个与天然病毒非常相似的颗粒 作为一种新颖的25 nm形式。我们现在已经在酵母中形成了颗粒 表达外壳蛋白和各种可包装的RNA 表达式构造。我们建议对这些粒子进行分析 用冷冻-高分辨扫描电子显微镜确定种群间的亲缘关系 病毒，T=3粒子，25 nm粒子，新粒子 它们不同的特性。

Virus-like particles have been found to assemble in yeast expressing brome mosaic virus coat protein due to viral replicative processes. Cryo - TEM and image reconstruction has shown the presence of 28 nm T=3 particles which strongly resemble native virus, as well as a novel 25 nm form. We have now formed particles in yeast by expressing the coat protein and various packagable RNAs from expression constructs. We propose to analyze these particle populations by Cryo-HRSEM to determine the relationships of true virus, the T=3 particles, the 25 nm particles, and the new particle to their varying properties.