CONTEMPORARY APPROACHES IN MOLECULAR/STRUCTURAL BIOLOGY

特拉华大学正在发起一项重大努力，并致力于 在校园里学习分子和结构生物学的重要资源。一个 外部寻找新的分子生物学家担任生物学主席 科学研究目前正在进行中，基金已承诺聘请一名 未来几年分子/结构生物学的新教员人数 好几年了。本创意基金的重点与我们的专业相辅相成。 推力与此职位直接相关，我们拟招聘 三个分子生物学系研究a)细胞信号，b) 利用转基因或基因敲除的发育、细胞周期或癌症生物学 鼠标技术，以及c)基因传递和靶向。此外，我们正在 建议聘请一位对蛋白质感兴趣的X射线结晶学家- 核酸相互作用。招募可能最好的候选人 在这些岗位上，我们正计划发展分子细胞生物学 设施和一个X射线结晶学实验室。一旦就位， 设施将对新员工和现有员工都有很大好处 致力于健康相关问题的教员。细胞生物学设施将 由三个主要部分组成：a)细胞培养实验室，参与 包括胚胎干细胞在内的各种细胞类型的生长和维持 将用于产生转基因和/或基因敲除的细胞 小鼠，b)用于导入的表达和载体工具 基因进入细胞，DNA测序，并促进生产 克隆的基因产物，以及c)单抗和免疫化学 将产生免疫试剂和研究细胞的设施，通过 用共聚焦显微镜确定抗原的分布或获得 X射线结晶学中心的结构和功能信息 将是一个由一名新教员指导的现代设施，以确定 重要生物分子的三维结构。我们预见到 新员工和增加的设施将导致一些新的NIH- 在特拉华州资助的项目在此的资助期结束时 格兰特。

The University of Delaware is initiating a major effort and committing significant resources into molecular and structural biology on campus. An external search for a new molecular biologist as Chair of Biological Sciences is presently underway and funds have been committed to hire a number of new faculty in molecular/structural biology over the next few years. The focus of the present IdeA grant complements well with our major thrust Directly related to this application, we are proposing to recruit three molecular biology faculty doing research on a) cell signaling, b) developmental, cell cycle, or cancer biology using transgenic or knock-out mouse technology, and c) gene delivery and targeting. In addition, we are proposing to hire an X-ray crystallographer who is interested in protein- nucleic acid interactions. To recruit the best possible candidates for these positions, we are planning to develop a molecular cell biology facility and an X-ray crystallography laboratory. Once in place, the facilities will be of enormous benefit to the new hires and to existing faculty working on health-related problems. The cell biology facility will consist of three major parts: a) a cell culture laboratory involved in the growth and maintenance of various cell types including embryonal stem cells that will be used for the generation of transgenic and/or knock-out mice, b) an expression and vector facility to be used for introducing genes into cells, sequencing DNA, and facilitating the production of cloned gene products, and c) a monoclonal antibody and immunochemistry facility that will generate immunological reagents and study cells by confocal microscopy to determine the distribution of antigen or to obtain structural and functional information The X-ray crystallography center will be a modem facility directed by a new faculty member to determine the three dimensional structure of important biomolecules. We envision that the new hires and the added facilities will result in a number of new NIH- funded projects at Delaware by the end of the funding period for this grant.