CONVERSION OF GLUCOSINOLATES TO ISOTHIOCYNATES IN SUBJECTS FED BROCCOLI SPROUTS

4名男性志愿者（43至53岁; 3名黑人，1名白色）， 入组、给药和监测。 在最初的三天洗脱期间， 在此期间，总二硫代氨基甲酸盐排泄量范围从检测不到到70.7 摩尔 然而，对于所有四名志愿者来说， 检测（0.124 mol/8小时），第一次检测前至少16小时 西兰花芽剂量。 在所有四个科目中， 制剂，尿二硫代氨基甲酸盐排泄量在前四个月达到峰值 除1例受试者外，所有受试者的排泄时间均为24小时， 在4-8小时的收集中达到峰值。尽管西兰花 发芽芥子油苷和异硫氰酸酯剂量相等（100摩尔）， 异硫氰酸盐制剂产生的尿液几乎是 二硫代氨基甲酸酯（89.6 ± 5.88mol，范围83.9 - 95.9）比 芥子油苷制剂（13.1 ± 1.65 mol，范围11.3 - 15.7）。 这 差异具有统计学显著性（p = 0.0003，双尾配对 t检验）。 尽管萝卜后总二硫代氨基甲酸盐排泄量 制剂明显超过剂量高达2.9倍，其 对西兰花异硫氰酸酯结果的贡献，占总量的3% 排泄，仍然可以忽略。

Four male volunteers (43 to 53 years old; 3 black, 1 white) were enrolled, dosed, and monitored. During the initial three day washout period, total dithiocarbamate excretion ranged from undetectable to 70.7 mol. However, for all four volunteers, levels fell below the limit of detection (0.124 mol/8 hour) by at least 16 hrs prior to the first broccoli sprout dose. In all four subjects, and for all three preparations, urinary dithiocarbamate excretion peaked in the first four hours for all but one subject, whose excretion after glucosinolates peaked in the 4-8 hour collection. Despite the fact that the broccoli sprout glucosinolates and isothiocyanates doses were equal (100 mol), the isiocyanate preparation yielded nearly seven times more urinary dithicarbamates (89.6 1 5.88 mol, range 83.9 - 95.9) than did the glucosinolate preparation (13.1 1 1.65 mol, range 11.3 - 15.7). This difference is statistically significant (p = 0.0003, two-tailed paired t-test). Although total dithiocarbamate excretion after the daikon preparation apparently exceeded the dose by a factor of up to 2.9, its contribution to the broccoli isothiocyanate results, at 3% of total excretion, remain negligible.