LASER MICROBEAM MANIPULATION OF CELL MORPHOGENESIS IN FUGAL HYHAE

真菌的一个特点是它们的体细胞形成 菌丝-长，两极分化，管状，有壁细胞，生长 在它们的尖端局部伸长。 菌丝顶端的生长是由 分泌囊泡和策略性排列的细胞骨架元件 集中在菌丝顶端，并假定提供 新质膜局部梯度所需的材料， 细胞壁随着细胞以高达2 嗯， 在隔膜（高等）真菌中，分泌囊泡， 微泡、细胞骨架成分和其他小细胞成分 被组织成一个动态的和可见的相-暗多组分 位于顶端的称为Spitzenkorper（Rtip bodies）的复合体 生长细胞的极。 存在与行为的协调 和细胞生长的模式清楚地表明 这个装置在细胞生长中起着重要的作用。 我们的目标 是为了帮助人们更好地伊势 亚细胞机制负责菌丝顶端生长，因为这 过程是真菌生长的主要机制， 环境. 获取重要线索的一个有用方法是 使细胞受到局部扰动， Spitzenkorper和其他选定的细胞成分。 为此，激光 微束捕获和烧蚀揭示了令人耳目一新的重要 在寻找发展机制方面取得了新的进展。

A hallmark of the fungi is that their somatic cells form hyphae--long, polarized, tubular, walled cells, which grow by localized elongation at their tips. Hyphal tip growth is mediated by secretory vesicles and strategically arranged cytoskeletal elements concentrated in the hyphal apex and are assumed to provide the materials needed for localized gradients of new plasma membrane and cell wall as the cell continuously elongates at rates as high a 2 um/s. In the sepate (higher) fungi, secretory vesicles, microvesicles, cytoskeletal elements, and other small cell components are organized into a dynamic and visible phase-dark multicomponent complex called the Spitzenkorper (Rtip bodyS) located at the apical pole of growing cells. Coordination between the presence and behavior of the Spitzenkorper and patterns of cell growth clearly demonstrates that this apparatus plays a significant role in cell growth. Our goal is to contribute to a detailed understanding of the prec ise subcellular mechanisms responsible for hyphal tip growth because this process is the predominant mechanism by which fungi grow in nearly all environments. A useful approach for obtaining significant clues is to subject the cells to localized perturbations which manipulate the Spitzenkorper and other selected cell components. To this end, laser microbeam trapping and ablation have revealed refreshing and important new advances in the search for developmental mechanisms.