ALTERATIONS IN ENDOTHELIAL CELL METABOLISM DURING HEART TRANSPLANT REJECTION

心脏移植现在是一种可以接受的治疗选择 适用于精选的终末期心脏病患者。五年 移植受者的存活率超过70%。不幸的是， 这些移植受者中有40%会患上移植冠状动脉 动脉疾病，一种弥漫性但通常残留的血管病变 直到它的后期状态才被察觉。它可能是一种慢性 血管排斥反应，当存在时，最终导致左 心功能不全和移植物失败。调查人员 计划测试这一假设，特别是血管组织 内皮细胞，会显示出代谢异常 心脏移植排斥反应，尽管保存了冠脉灌流。这个 将使用多指示剂稀释技术来评估腺苷 分离的大鼠异位心脏移植准备中的摄取。 研究人员将比较血管内皮细胞腺苷流量 同种异体心脏移植对排斥反应的控制 同种异体心脏移植腺苷流量也将在一组 大鼠异位心脏移植的实验研究 环孢素和甲基强的松龙。

Heart transplantation is now an acceptable treatment alternative for select patients with end-stage heart disease. The five year survival for transplant recipients is over 70%. Unfortunately up to 40% of these transplant recipients will develop transplant coronary artery disease, a vasculopathy which is diffuse but generally remains undetected until its late states. It may be a form of chronic vascular rejection and when present, ultimately results in left ventricular dysfunction and failure of the graft. The investigators plan to test the hypothesis that vascular tissue, in particular endothelial cells, will reveal abnormalities in metabolism during heart transplant rejection, despite preserved coronary perfusion. The multiple indicator dilution technique will be used to assess adenosine uptake in an isolated rat heterotopic heart transplant preparation. The investigators will compare the endothelial adenosine flux in control syngeneic heart allografts to that of allogeneic rejecting heart allografts. Adenosine flux will also be assessed in a group of allogeneic heterotopic heart transplants from rats treated with cyclosporine and methylprednisolone.