RECONSTRUCTION OF SPIROCHETE 3D MORPHOLOGY: PERIODONTAL DIS, LYME

（由NIH K11DE00257和NIH RO1 AI29743至N. Charon）。 导致牙周病的螺旋体，密螺旋体 有独特的形态。 最外面是膜鞘， 并且在该鞘内是螺旋形的蜂窝状圆柱体。 在 细胞圆柱体的每一端，细菌鞭毛样细胞器， 被称为周质鞭毛。 这些周质鞭毛 存在于外膜鞘之间的周质空间， 细胞圆柱体。 遗传分析表明这些细胞器 与螺旋体的运动有关 此外，几个 有证据表明，这些周质鞭毛推动了 生物通过旋转的方式类似于伯氏疏螺旋体，莱姆 疾病螺旋体。 我们知道周质鞭毛不仅 在运动中发挥作用，但影响细胞的形状 他们居住的地方。 使用HVEM立体对，我们研究了 周质鞭毛在生物体内的配置：如何 它们围绕蜂窝圆柱体缠绕，以及在哪个方向上，即， 顺时针和逆时针。 根据我们得到的结果，我们 了解了这些细胞器如何影响 有机体。 Goldstein，S.F.，K.F. Buttle，and N.W. Charon. （迟交） 1996）钩端螺旋体科和疏螺旋体的结构分析 burgdorferi的高压电子显微镜观察。 J. Bact.， 178：6539 - 6545。 鲁比，J.D.，H. Li，H. Kuramitsu，S.J. Norris，S.F. Goldstein，K.F.巴特尔和西北卡戎。 1997年：关系 齿垢密螺旋体周质鞭毛至不规则细胞形态。 J.细菌，179：1628 - 1635。

(Supported in part by NIH K11DE00257 and NIH RO1 AI29743 to N. Charon). The spirochete that causes periodontal disease, Treponema denticola, has a unique morphology. Outer most is a membrane sheath, and within this sheath are a helical shaped Cellular cylinder. At each end of the Cellular cylinder, bacterial flagella-like organelles, called periplasmic flagella are attached. These periplasmic flagella reside in the periplasmic space between the outer membrane sheath and Cellular cylinder. Genetic analysis has shown that these organelles are involved in the motility of spirochetes. In addition, several lines of evidence indicate that these periplasmic flagella propel the organisms by rotation in manner similar Borrelia burgdorferi, the lyme disease spirochete. We know that the periplasmic flagella not only play a role in motility, but influence the shape of the cellular domain where they reside. Using HVEM stereo pairs, we studied the configuration of the periplasmic flagella within the organism: How they wrap around the Cellular cylinder, and in which direction, i.e. clockwise and counter clockwise. Based on the results we obtained, we learned how these organelles influence the shape and the motility of the organisms. Goldstein, S.F., K.F. Buttle, and N.W. Charon. (Late 1996) Structural Analysis of the Leptospiraceae and borrelia burgdorferi by high-voltage electron microscopy. J. Bact., 178:6539-6545. Ruby, J.D., H. Li, H. Kuramitsu, S.J. Norris, S.F. Goldstein, K.F. Buttle and N.W. Charon. (1997) Relationship of Treponema denticola periplasmic flagella to irregular cell morphology. J. Bact., 179:1628-1635.